Organization and sequence of the HpaII restriction-modification system and adjacent genes
1994; Elsevier BV; Volume: 142; Issue: 1 Linguagem: Inglês
10.1016/0378-1119(94)90348-4
ISSN1879-0038
AutoresSaulius Kulakauskas, Janet M. Barsomian, Arvydas Lubys, Richard J. Roberts, Geoffrey G. Wilson,
Tópico(s)RNA and protein synthesis mechanisms
ResumoWe report the organization of the HpaII restriction and modification (R-M) system from Haemophilus parainfluenzae (recognition sequence: 5'…CCGG…3'), the sequence of the gene coding for the HpaII restriction endonuclease, and the sequence of the upstream flanking DNA. The HpaII system comprises two genes, hpaIIM, coding for the methyltransferase (MTase; 358 amino acids (aa), 40.4 kDa: product, Cm5CGG), and hpaIIR, coding for the restriction endonuclease (ENase; 358 aa, 40.9 kDa: product, C'CGG). The genes are adjacent, they have the same orientation, and they occur in the order hpaIIM then hpaIIR. The ENase bears little aa sequence similarity to the isoschizomeric R-BsuFI and R · MspI ENases. Upstream of, and partly overlapping hpaIIM is the coding sequence for a 141-aa protein that resembles the very-short-patch-repair endonuclease (Vsr) of Escherichia coli. Upstream ofthat is the coding sequence for a protein that resembles valyl-tRNA synthetase (ValS).
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