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MORPHOGENESIS OF ISOLATED FLORAL BUDS OF RANUNCULUS SCELERATUS L. IN VITRO

1969; Thieme Medical Publishers (Germany); Volume: 18; Issue: 6 Linguagem: Inglês

10.1111/j.1438-8677.1969.tb00091.x

1365-2001

R. N. Konar, Karaba N. Nataraja,

Chromosomal and Genetic Variations

Acta Botanica NeerlandicaVolume 18, Issue 6 p. 680-699 Article MORPHOGENESIS OF ISOLATED FLORAL BUDS OF RANUNCULUS SCELERATUS L. IN VITRO R. N. Konar, R. N. Konar Department of Botany, University of Delhi, Delhi 7, IndiaSearch for more papers by this authorK. Nataraja, K. Nataraja Department of Botany, University of Delhi, Delhi 7, IndiaSearch for more papers by this author R. N. Konar, R. N. Konar Department of Botany, University of Delhi, Delhi 7, IndiaSearch for more papers by this authorK. Nataraja, K. Nataraja Department of Botany, University of Delhi, Delhi 7, IndiaSearch for more papers by this author First published: December 1969 https://doi.org/10.1111/j.1438-8677.1969.tb00091.xCitations: 24AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat SUMMARY Morphogenesis of floral buds excised at various stages of development was followed in vitro. The buds comprising the primordia of sepals and stamens (Stage I) failed to complete normal development on any of the nutrient media tried. However, the initiation and further development of carpels occurred even on a medium containing mineral elements, glycine, vitamins and sucrose. On the other hand, the buds having the anthers at the pollen mother cell stage (Stage II) completed microsporogenesis and 2-celled pollen grains were formed in the anthers. The torus i.e. the central dome bearing the carpels, of Stage II, and III (buds having anthers at pollen grain stage) elongated enormously and emerged through the folded sepals. Regeneration of roots and shoot buds (especially in Stage II & III buds) was common. In addition, the floral buds of all stages formed callus which subsequently differentiated roots, shoot buds and embryoids leading to the formation of plantlets. The latter, in turn, developed embryoids from the epidermal and pith cells of the stem. On subculturing, the hypocotyl portion of the in vitro plantlet was capable of developing embryoids directly from the epidermal cells, whereas the radicular and plumular portions of the same plantlet first formed callus and subsequently embryoids. The growth of callus and the differentiation of embryoids could be maintained through repeated subculturing. Embryoids could be induced even on a simple medium having only mineral elements and sucrose. Among the several growth adjuvants used, a combination of coconut milk and IAA supported best callus growth and normal embryoid differentiation. Citing Literature Volume18, Issue6December 1969Pages 680-699 RelatedInformation