Portal de Periódicos da CAPES

The structure of CMP:2-keto-3-deoxy- manno -octonic acid synthetase and of its complexes with substrates and substrate analogs 1 1Edited by I. A. Wilson

2001; Elsevier BV; Volume: 312; Issue: 1 Linguagem: Inglês

10.1006/jmbi.2001.4948

1089-8638

S. Jelakovic, Georg E. Schulz,

Microbial Natural Products and Biosynthesis

The enzyme CMP-Kdo synthetase (CKS) catalyzes the activation of the sugar Kdo (2-keto-3-deoxy-manno-octonic acid) by forming a monophosphate diester. CKS is a pharmaceutical target because CMP-Kdo is used in the biosynthesis of lipopolysaccharides that are vital for Gram-negative bacteria. We have refined the structure of the unligated capsule-specific CKS from Escherichia coli at 1.8 Å resolution (1 Å=0.1 nm) and we have established the structures of its complexes with the substrate CTP, with CDP and CMP as well as with the product analog CMP-NeuAc (CMP-sialate) by X-ray diffraction analyses at resolutions between 2.1 Å and 2.5 Å. The N-terminal domains of the dimeric enzyme bind CTP in a peculiar nucleotide-binding fold, whereas the C-terminal domains form the dimer interface. The observed binding geometries together with the amino acid variabilities during evolution and the locations of a putative Mg2+ and of a very strongly bound water molecule suggest a pathway for the catalysis. The N-terminal domain shows sequence homology with the CMP-NeuAc synthetases. Moreover, the chain fold and the substrate-binding position of CKS resemble those of other enzymes processing nucleotide-sugars.