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European multicentre evaluation of a commercial system for identification of methicillin-resistantStaphylococcus aureus

1996; Springer Science+Business Media; Volume: 15; Issue: 9 Linguagem: Inglês

10.1007/bf01691964

1435-4373

Gilles Zambardi, J Fleurette, Gian Carlo Schito, R Auckenthaler, E Bergogne-Bérézin, R. Hone, Anna King, W. Lenz, C Lohner, A. Makristhatis, Francesc Marco, Claudette Muller‐Serieys, C. Nonhoff, Ian Phillips, Peter Rohner, M. Rotter, K. P. Schaal, Marc Struelens, A. Viebahn,

Streptococcal Infections and Treatments

A commercial system for the rapid detection of methicillin-resistant Staphylococcus aureus, the BBL Crystal MRSA test (C-MRSA ID; Becton Dickinson, USA), was evaluated prospectively and compared with a polymerase chain reaction test for the presence of the mecA gene. Ten European centres tested a total of 676 isolates of Staphylococcus aureus from blood cultures. The system correctly identified 661 (97.8%) isolates within 4 h. All but three mecA gene-negative isolates (99.4% specificity) yielded a negative C-MRSA ID reaction, and 158 of 170 mecA gene-positive isolates were accurately detected (92.9% sensitivity). After repeated testing of discrepant results, sensitivity and specificity increased to 99% and 100%, respectively.