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Replacement of the deletion in the genome (0.762–0.789 mu) of avirulent HSV-1 HFEM using cloned Mlul DNA fragment (0.7615–0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant

1986; Elsevier BV; Volume: 5; Issue: 2-3 Linguagem: Inglês

10.1016/0168-1702(86)90015-8

1872-7492

Angela Rösen, Fedor Ernst, Hans-Georg Koch, Hans Gelderblom, Gholamreza Darai, Julia Hadar, Eynat Tabor, Tamir Ben‐Hur, Yechiel Becker,

Polyomavirus and related diseases

The HFEM strain of HSV-1 is apathogenic for the tree shrew by the intraperitoneal (i.p.) route because of a deletion in the genome coordinates 0.762-0.789. Insertion of the MluI DNA fragment (coordinates 0.7615-0.789) cloned from HSV-1 strain F, which is pathogenic for the tree shrew, restored the i.p. pathogenicity to strain HFEM. The recombinant designated R-M1-C1 was highly pathogenic for the tree shrew, but slightly virulent for inbred mouse strain A. It thus appears that the viral DNA sequence involved in the i.p. pathogenicity of HSV-1 is located within the genome coordinates 0.761-0.796. This sequence is recognized differently by the cellular elements involved in HSV-1 infection in the tree shrew and the mouse.