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The effect of peptide surface density on mineralization of a matrix deposited by osteogenic cells

2000; Wiley; Volume: 52; Issue: 4 Linguagem: Inglês

10.1002/1097-4636(20001215)52

1097-4636

Alireza Rezania, Kevin E. Healy,

Polymer Surface Interaction Studies

Journal of Biomedical Materials ResearchVolume 52, Issue 4 p. 595-600 The effect of peptide surface density on mineralization of a matrix deposited by osteogenic cells Alireza Rezania, Alireza Rezania Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008 Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60201Search for more papers by this authorKevin E. Healy, Corresponding Author Kevin E. Healy [email protected] Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008 Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60201Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008Search for more papers by this author Alireza Rezania, Alireza Rezania Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008 Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60201Search for more papers by this authorKevin E. Healy, Corresponding Author Kevin E. Healy [email protected] Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008 Department of Biomedical Engineering, Robert R. McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60201Division of Biological Materials, Northwestern University, 311 E. Chicago Avenue, Chicago, Illinois 60611-3008Search for more papers by this author First published: 03 October 2000 https://doi.org/10.1002/1097-4636(20001215)52:4 3.0.CO;2-3Citations: 147AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The density of Arg-Gly-Asp-containing peptides covalently grafted to solid materials has been shown to affect adhesion, spreading, and focal contact formation. The objective of this study was to examine the effect of ligand density on mineralization of the extracellular matrix deposited by osteoblasts. In particular, RGD-modified quartz surfaces with ligand densities varying over two orders (0.01–3.6 pmol/cm2) of magnitude were prepared to assess the long-term function of osteoblasts on peptide-derivatized surfaces. After 3 weeks in culture, surfaces modified with a 15 amino acid peptide (Ac-Cys-Gly-Gly-Asn-Gly-Glu-Pro-Arg-Gly-Asp-Thr-Tyr-Arg-Ala-Tyr-NH2) at a density ≥0.62 pmol/cm2 significantly (p < 0.05) enhanced mineralization compared with a RGD surface density of 0.01 pmol/cm2, RGE surfaces, or clean surfaces adsorbed with serum proteins. These results suggest that regulation of the surface density of adhesive ligands on biomaterial surfaces is a critical determinant in a strategy to alter the degree of extracellular matrix maturation in contact with solid surfaces (e.g., implants). 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