Chromatographic separation and identification of chloroplast pigments in Chlorella pyrenoidosa
1967; Elsevier BV; Volume: 31; Linguagem: Inglês
10.1016/s0021-9673(01)86125-2
ISSN1873-3778
Autores Tópico(s)Chromatography in Natural Products
ResumoThis chapter covers the subject of instrumental thin-layer chromatographic (TLC) analysis of plant extracts. Single development techniques for the separation of plant extract fractions of various structures are reviewed. The following metabolite groups are described: polyphenolic compounds (phenolic acids, flavonoids, coumarins, lignans), terpenoids (mono-, di-, sesqui- terpenes, saponins, carotenoids), iridoids, alkaloids, and quinones. Special techniques for chromatogram development, such as: unidimensional multiple development, incremental multiple development, and bivariant multiple development and their application in plant analysis are described. Multidimensional separations and their realization by TLC are described, e.g., 2D-TLC on one plate, on bilayers, and on connected plates (graft TLC) with examples of their use in phytochemical analysis. Strategies for the separation of compounds of a wide polarity range, such as stepwise gradient development, binary development, and multiple gradient development are addressed. Also the strategies for separation of structural analogues are mentioned. The use of all the aforementioned techniques in fingerprint analysis of plant material is discussed. The advantages of using biological detection for screening of plant samples for the presence of pharmacologically active metabolites are characterized. Finally the use of image processing of TLC videoscans or photographs is mentioned.
Referência(s)