Capillary electrophoresis—matrix-assisted laser-desorption ionization mass spectrometry of proteins
1994; Elsevier BV; Volume: 680; Issue: 2 Linguagem: Inglês
10.1016/0021-9673(94)85131-x
ISSN1873-3778
AutoresWolfgang Weinmann, Carol E. Parker, Leesa J. Deterding, Damon I. Papac, John Hoyes, Michael Przybylski, Kenneth B. Tomer,
Tópico(s)Analytical Chemistry and Chromatography
ResumoAn “off-line” combination of capillary electrophoresis (CE) with matrix-assisted laser-desorption mass spectrometry (MALDI-MS) has been developed for the structural characterization of CE-separated peptides and proteins. Using a sheath flow interface, similar to that developed for “on-line” CE—fast atom bombardment MS and CE—electrospray MS, an efficient sample isolation procedure has been developed which is applicable to bioorganic compounds in aqueous buffer solutions. This isolation procedure, with subsequent transfer to the MALDI-MS sample target, has been successfully used for the direct analysis of CE-separated proteins of Mr up to 67 000, and a mixture of apolipoprotein AII monomer and homodimer, using sample amounts of less than 1 pmol.
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