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Studies on the nature of messenger RNA in phytohemagglutinin-stimulated rat lymphocytes

1971; Elsevier BV; Volume: 254; Issue: 3 Linguagem: Inglês

10.1016/0005-2787(71)90877-x

1879-3002

Gideon Berke, S Sarid, M. Feldman,

Acute Lymphoblastic Leukemia research

Ribonucleic acid synthesis during phytohemagglutinin-induced transformation of rat lymphocytes was investigated by deoxyribonucleic acid · ribonucleic acid hybridization and hybridization competition. It was found that the amount of ribonucleic acid complementary to deoxyribonucleic acid varied as a function of time after stimulation of lymphocytes with phytohemagglutinin. Ribonucleic acid synthesized between 19 and 24 h in stimulated lymphocytes showed the lowest hybridizability (approx. 2–3 % of the deoxyribonucleic acid), whereas ribonucleic acid synthesized either before or after this period showed a greater extent of hybridization. Hybridization-competition experiments demonstrated that unlabeled small-lymphocyte ribonucleic acid was a successful competitor in the reaction between radioactive transformed-lymphocyte ribonucleic acid and lymphocyte deoxyribonucleic acid. This suggests that resting small lymphocytes and transformed lymphocytes possess similar species of ribonucleic acid.