Mechanistic Basis for the Action of New Cephalosporin Antibiotics Effective against Methicillin- and Vancomycin-resistant Staphylococcus aureus
2006; Elsevier BV; Volume: 281; Issue: 15 Linguagem: Inglês
10.1074/jbc.m508846200
ISSN1083-351X
AutoresCosimo Fuda, Dušan Hesek, Mijoon Lee, Werner Heilmayer, Rodger Novak, Sergei B. Vakulenko, Shahriar Mobashery,
Tópico(s)Bacterial biofilms and quorum sensing
ResumoEmergence of methicillin-resistant Staphylococcus aureus (MRSA) has created challenges in treatment of nosocomial infections. The recent clinical emergence of vancomycin-resistant MRSA is a new disconcerting chapter in the evolution of these strains. S. aureus normally produces four PBPs, which are susceptible to modification byβ-lactam antibiotics, an event that leads to bacterial death. The gene product of mecA from MRSA is a penicillin-binding protein (PBP) designated PBP 2a. PBP 2a is refractory to the action of all commercially available β-lactam antibiotics. Furthermore, PBP 2a is capable of taking over the functions of the other PBPs of S. aureus in the face of the challenge by β-lactam antibiotics. Three cephalosporins (compounds 1–3) have been studied herein, which show antibacterial activities against MRSA, including the clinically important vancomycin-resistant strains. These cephalosporins exhibit substantially smaller dissociation constants for the preacylation complex compared with the case of typical cephalosporins, but their pseudo-second-order rate constants for encounter with PBP 2a (k2/Ks) are not very large (≤200 m–1 s–1). It is documented herein that these cephalosporins facilitate a conformational change in PBP 2a, a process that is enhanced in the presence of a synthetic surrogate for cell wall, resulting in increases in the k2/Ks parameter and in more facile enzyme inhibition. These findings argue that the novel cephalosporins are able to co-opt interactions between PBP 2a and the cell wall in gaining access to the active site in the inhibition process, a set of events that leads to effective inhibition of PBP 2a and the attendant killing of the MRSA strains. Emergence of methicillin-resistant Staphylococcus aureus (MRSA) has created challenges in treatment of nosocomial infections. The recent clinical emergence of vancomycin-resistant MRSA is a new disconcerting chapter in the evolution of these strains. S. aureus normally produces four PBPs, which are susceptible to modification byβ-lactam antibiotics, an event that leads to bacterial death. The gene product of mecA from MRSA is a penicillin-binding protein (PBP) designated PBP 2a. PBP 2a is refractory to the action of all commercially available β-lactam antibiotics. Furthermore, PBP 2a is capable of taking over the functions of the other PBPs of S. aureus in the face of the challenge by β-lactam antibiotics. Three cephalosporins (compounds 1–3) have been studied herein, which show antibacterial activities against MRSA, including the clinically important vancomycin-resistant strains. These cephalosporins exhibit substantially smaller dissociation constants for the preacylation complex compared with the case of typical cephalosporins, but their pseudo-second-order rate constants for encounter with PBP 2a (k2/Ks) are not very large (≤200 m–1 s–1). It is documented herein that these cephalosporins facilitate a conformational change in PBP 2a, a process that is enhanced in the presence of a synthetic surrogate for cell wall, resulting in increases in the k2/Ks parameter and in more facile enzyme inhibition. These findings argue that the novel cephalosporins are able to co-opt interactions between PBP 2a and the cell wall in gaining access to the active site in the inhibition process, a set of events that leads to effective inhibition of PBP 2a and the attendant killing of the MRSA strains. Staphylococcus aureus was exquisitely sensitive to penicillins in the early years of the use of β-lactams in the clinic. In the 1940s, resistance to first generation penicillins emerged from a profusion of strains containing a class A β-lactamase. In response to the β-lactamase challenge, a second generation of penicillins that included methicillin was introduced in 1959. By 1961, a strain of S. aureus emerged first in the United Kingdom and subsequently in other parts of the world, which was resistant to methicillin and other β-lactams. This strain became known as methicillin-resistant S. aureus (MRSA). 2The abbreviations used are: MRSA, methicillin-resistant S. aureus; PBP, penicillin-binding protein; FL, fluorescent; MIC, minimum inhibitory concentration. 2The abbreviations used are: MRSA, methicillin-resistant S. aureus; PBP, penicillin-binding protein; FL, fluorescent; MIC, minimum inhibitory concentration. The recent emergence of variants of MRSA that are resistant to glycopeptide antibiotics, such as vancomycin, due to the acquisition of the vanA gene from Enterococci, has created a new and disconcerting chapter in the evolution of this organism. MRSA is one of the most serious infectious disease agents globally. The need for novel antibiotics to treat this organism is genuine. The basis for MRSA resistance to β-lactam antibiotics is complex (1Gardete S. Ludovice A.M. Sobral R.G. Filipe S.R. De Lencastre H. Tomasz A. J. Bacteriol. 2004; 186: 1705-1713Crossref PubMed Scopus (37) Google Scholar, 2Sobral R.G. Ludovice A.M. Gardete S. Tabei K. De Lencastre H. Tomasz A. Microb. Drug Resist. 2003; 9: 231-241Crossref PubMed Scopus (42) Google Scholar, 3Katayama Y. Zhang H.Z. Hong D. Chambers H.F. J. Bacteriol. 2003; 185: 5465-5472Crossref PubMed Scopus (76) Google Scholar, 4Garcia-Castellanos R. Mallorqui-Fernandez G. Marrero A. Potempa J. Coll M. Gomis-Ruth F.X. J. Biol. Chem. 2004; 279: 17888-17896Abstract Full Text Full Text PDF PubMed Scopus (58) Google Scholar, 5Katayama Y. Zhang H.Z. Chambers H.F. Antimicrob. Agents Chemother. 2004; 48: 453-459Crossref PubMed Scopus (63) Google Scholar). Nonetheless, a primary factor is the acquisition of the gene mecA, which encodes a penicillin-binding protein (PBP) designated as PBP 2a. Whereas β-lactam antibiotics can inhibit their target PBPs in penicillin-sensitive S. aureus strains, a process that impairs the final steps of cell wall biosynthesis and leads to bacterial death, PBP 2a is refractory to inhibition by all commercially available β-lactam antibiotics (5Katayama Y. Zhang H.Z. Chambers H.F. Antimicrob. Agents Chemother. 2004; 48: 453-459Crossref PubMed Scopus (63) Google Scholar, 6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar, 7Chambers H.F. Trends Microbiol. 2003; 11: 145-148Abstract Full Text Full Text PDF PubMed Scopus (33) Google Scholar, 8Diekema D.J. Pfaller M.A. Schmitz F.J. Smayevski J. Bell J. Jones R.N. Beach M. Clin. Infect. Dis. 2001; 32: S114-S132Crossref PubMed Scopus (1091) Google Scholar). Hence, whereas β-lactam antibiotics inhibit the regular S. aureus PBPs, the β-lactam-insensitive PBP 2a takes over their biosynthetic functions. Hence, MRSA acquires a survival advantage to the challenge by β-lactam antibiotics. The basis for PBP 2a resistance to inhibition is complex. The rate constant for acylation of the active site serine by the β-lactam is attenuated, and the dissociation constant for the preacylation β-lactam-enzyme complex is less favorable (6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar, 9Lu W.P. Sun Y. Bauer M.D. Paule S. Koenigs P.M. Kraft W.G. Biochemistry. 1999; 38: 6537-6546Crossref PubMed Scopus (61) Google Scholar). An additional reason for PBP 2a β-lactam resistance would appear to be the lack of access of the β-lactam to its active site. The active site of PBP 2a is normally closed and must open to bind to the bacterial cell wall, its substrate (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar, 11Lim D. Strynadka N.C. Nat. Struct. Biol. 2002; 9: 870-876PubMed Google Scholar). We recently documented that in concert with interactions with cell wall fragments outside the active site, the active site becomes more accessible by a conformational change (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). The clinical difficulties in treatment of MRSA have prompted the pharmaceutical industry to search for new β-lactams active against the PBP 2a of MRSA (12Bogdanovich T. Ednie L.M. Shapiro S. Appelbaum P.C. Antimicrob. Agents Chemother. 2005; 49: 4210-4219Crossref PubMed Scopus (164) Google Scholar, 13Iizawa Y. Nagai J. Ishikawa T. Hashiguchi S. Nakao M. Miyake A. Okonogi K. J. Infect. Chemother. 2004; 10: 146-156Abstract Full Text PDF PubMed Scopus (83) Google Scholar, 14Vouillamoz J. Entenza J.M. Hohl P. Moreillon P. Antimicrob. Agents Chemother. 2004; 48: 4322-4327Crossref PubMed Scopus (14) Google Scholar). We report herein our analyses with three novel cephalosporins, 1–3 (Fig. 1), discovered at the Sandoz Antibiotic Research Institute of Vienna. Cephalosporin 1 is currently undergoing clinical trials for treatment of MRSA infections. These cephalosporins are found to have significantly smaller dissociation constants for the preacylation complex with PBP 2a compared with other β-lactam antibiotics, as will be discussed. However, the rate constants for the PBP 2a acylation by these three are similar to those for other β-lactams. Moreover, the rate constants for PBP 2a acylation by these cephalosporins are enhanced considerably in the presence of an analogue of a fragment of bacterial cell wall (compound 4). This suggests that these cephalosporins might be co-opting interactions of the cell wall with PBP 2a in vivo to gain access to the active site of this enzyme in the course of covalent inhibition that leads to bacterial death. The cloning and purification of soluble PBP 2a has been described (6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar, 10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). Determination of the Kinetic Parameters for Interactions of Cephalosporins with PBP 2a—The first-order rate constants for the interactions between the cephalosporins 1–3 and PBP 2a of MRSA were evaluated using a Cary 50 UV spectrophotometer (Varian Inc.) at room temperature. The reaction between PBP 2a and nitrocefin was monitored by the formation of the acyl-enzyme species at 500 nm (Δϵ500 =+15,900 cm–1 m–1). The experiments were carried out in 25 mm Hepes, 1 m NaCl (pH 7.0) buffer. Nitrocefin (120 μm) was used as the reporter molecule to determine the apparent first-order rate constants for acylation by the nonchromogenic (or poorly chromogenic) cephalosporins 1–3 at varying concentrations (10–120 μm) in the presence and absence of compound 4 (0.5–2 mm) by the methods of Graves-Woodward et al. (15Graves-Woodward K. Pratt R.F. Biochem. J. 1998; 332: 755-761Crossref PubMed Scopus (48) Google Scholar). Compounds 1–3 were dissolved in 25 mm Hepes, 1 m NaCl (pH 7.0) buffer. UV-spectroscopic analysis of the compounds in the range of 200–350 nm at room temperature for 4 days indicated no change, consistent with the stability of the antibiotics within this duration. The deacylation rate constants (k3) were determined using BOCILLIN FL as a reporter molecule. A typical reaction mixture (60 μl) contained 15 μm PBP 2a and cephalosporin 1, 2, or 3 at a concentration 2-fold higher than its Kd value. The mixture was incubated at room temperature for 45 min in 25 mm Hepes, 1 m NaCl (pH 7.0) buffer. The excess cephalosporin was removed by passing the mixture through a Micro Bio-Spin®6 column (Bio-Rad). Deacylation rate constants for cephalosporins 1–3 were determined both in the presence and absence of the cell wall surrogate compound 4. In the experiments in the presence of compound 4, it (1.5 mm) was added to the resultant mixture immediately following the removal of excess antibiotic by passing the mixture through a Micro Bio-Spin®6 column (Bio-Rad). An aliquot (3 μl) of the mixture was diluted 5-fold with the same buffer and incubated at room temperature for different time intervals. The amount of free protein, liberated from the acyl-enzyme species, was assayed by the addition of BOCILLIN FL to afford a final concentration of 40 μm and incubated for an additional 20 min at room temperature. SDS sample buffer (15 μl) was added to the reaction mixture, which was then boiled for 3 min. The samples (30 μl in total) were loaded onto a 10% SDS-polyacrylamide gel, which was developed and then scanned using a Storm840® Fluorimager. Circular Dichroism—The CD spectra of PBP 2a (3 μm in 25 mm Hepes, 1 m NaCl, pH 7.0) were recorded on a stopped-flow circular dichroism spectrometer (AVIV INSTRUMENTS INC-202 SF) with a 2-mm path length in the absence and presence of a 6 μm concentration of each of the cephalosporins 1–3 at 25 °C. The nearly negligible contribution of each cephalosporin by itself was subtracted in each case. The ensuing conformational changes were then monitored for 96 h. Bacteria—The strain panel was comprised of S. aureus COL, S. aureus COLVA, S. aureus VRS1 isolated in Michigan (16Bartley J. Infect. Control Hosp. Epidemiol. 2002; 23: 480Crossref PubMed Google Scholar), and S. aureus VRS2 isolated in Pennsylvania (17Center for Disease Control and Prevention Morb. Mortal. Wkly. Rep. 2002; 51: 931PubMed Google Scholar). All four strains were obtained from the Network on Antimicrobial Resistance in Staphylococcus aureus. Also screened was a clinical isolate of vancomycin-resistant Enterococcus faecalis (E. faecalis 99) obtained from Wayne State University Medical Center. Susceptibility Testing—MICs were determined four times in each case by a broth microdilution method using cation-adjusted Mueller-Hinton II broth and procedures from the National Committee for Clinical Laboratory Standards (NCCLS) (18National Committee for Clinical Laboratory Standards Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. 6th Ed. National Committee for Clinical Laboratory Standards, Wayne, PA2003Google Scholar). Solubility of the Acyl-enzyme Species of Cephalosporins 1–3 and PBP 2a—A typical reaction mixture (100 μl) contained 9 μm PBP 2a and 20 μm cephalosporin 1, 2, or 3. The mixture was incubated at room temperature for 30 min in 25 mm Hepes, 1 m NaCl (pH 7.0) buffer. Aliquots (8 μl) of the mixture were diluted 3-fold with the same buffer and incubated for different time intervals (0–96 h). The samples were centrifuged (14,000 rpm) to pellet any protein that might have precipitated (none was visually detected). SDS sample buffer (15 μl) was added to the supernatant, and the mixture was boiled for 3 min and loaded onto a 10% SDS-polyacrylamide gel. PBP 2a (3 μm), in the absence of the cephalosporins, was run alongside as a control. The gel was treated with Coomassie Blue, destained, and scanned (Epson Perfection, 3200 Photo). The density of each PBP 2a band corresponding to a different time point was determined using ImageJ software. The results for each cephalosporin indicated that the acyl-enzyme complex remained fully soluble over the course of 4 days. Nondenaturing PAGE of the Acyl-enzyme Species of Cephalosporins 1–3 and PBP 2a—A typical reaction mixture (60 μl) contained 9 μm PBP 2a and 20 μm cephalosporins 1–3 in 25 mm Hepes, 1 m NaCl (pH 7.0) buffer. Aliquots (8 μl) of the mixture were diluted 3-fold with the same buffer and incubated for different time intervals (0–96 h). Nondenaturing sample buffer (15 μl) was added to aliquots of the sample, loaded onto a 5% nondenaturing polyacrylamide gel, and run alongside two controls, PBP 2a (3 μm) by itself and PBP 2a (3 μm) in the presence of ceftazidime (15 μm). Gels were run at 4 °C after 3, 24, and 96 h to monitor the occurrence of any oligomerization within the three samples. Each gel was treated with Coomassie Blue, destained, and scanned (Epson Perfection, 3200 Photo). The density of each PBP 2a band corresponding to the acyl-PBP 2a species (in the presence of cephalosporins 1–3) was compared with the two controls. There was no difference in the appearance of the bands for PBP 2a bound to antibiotics 1–3, for PBP 2a bound to ceftazidime, or for PBP 2a by itself. The results indicated that the acyl-enzyme species for each antibiotic or PBP 2a by itself did not form oligomers over the course of 4 days. Inhibition of PBPs by β-lactams follows this kinetic scheme. E+I⇄KsEI→k2E-I→k3E+P(Eq. 1) In this minimal scheme, E represents PBP 2a, EI is the noncovalent preacylation complex, E-I is the covalent acyl-enzyme species, and P denotes the product of E-I hydrolysis and restoration of enzyme activity. In the case of a typical PBP, the acylation rate constant (k2) is rapid, but deacylation (k3) is slow (19Jamin M. Hakenbeck R. Frere J.M. FEBS Lett. 1993; 331: 101-104Crossref PubMed Scopus (38) Google Scholar, 20Pagliero E. Chesnel L. Hopkins J. Croize J. Dideberg O. Vernet T. Di Guilmi A.M. Antimicrob. Agents Chemother. 2004; 48: 1848-1855Crossref PubMed Scopus (40) Google Scholar, 21Di Guilmi A.M. Mouz N. Andrieu J.P. Hoskins J. Jaskunas S.R. Gagnon J. Dideberg O. Vernet T. J. Bacteriol. 1998; 180: 5652-5659Crossref PubMed Google Scholar, 22Mouz N. Gordon E. Di Guilmi A.M. Petit I. Petillot Y. Dupont Y. Hakenbeck R. Vernet T. Dideberg O. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 13403-13406Crossref PubMed Scopus (75) Google Scholar). As the results with nitrocefin (a chromogenic cephalosporin), cefepime, and ceftazidime (two clinically used cephalosporins) with PBP 2a indicate (Table 1), the acylation rate constants are slower, by over 4 orders of magnitude, than the corresponding values for β-lactam-sensitive PBPs (19Jamin M. Hakenbeck R. Frere J.M. FEBS Lett. 1993; 331: 101-104Crossref PubMed Scopus (38) Google Scholar, 20Pagliero E. Chesnel L. Hopkins J. Croize J. Dideberg O. Vernet T. Di Guilmi A.M. Antimicrob. Agents Chemother. 2004; 48: 1848-1855Crossref PubMed Scopus (40) Google Scholar, 21Di Guilmi A.M. Mouz N. Andrieu J.P. Hoskins J. Jaskunas S.R. Gagnon J. Dideberg O. Vernet T. J. Bacteriol. 1998; 180: 5652-5659Crossref PubMed Google Scholar, 22Mouz N. Gordon E. Di Guilmi A.M. Petit I. Petillot Y. Dupont Y. Hakenbeck R. Vernet T. Dideberg O. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 13403-13406Crossref PubMed Scopus (75) Google Scholar, 23Lu W.P. Kincaid E. Sun Y. Bauer M.D. J. Biol. Chem. 2001; 276: 31494-31501Abstract Full Text Full Text PDF PubMed Scopus (50) Google Scholar). The deacylation rate constants (k3) are slower yet, with t½ of 26–77 h. Furthermore, the dissociation constants (Ks) for the preacylation complexes are elevated to values that preclude in vivo saturation of PBP 2a. Hence, these β-lactams (as with other examples) are ineffective PBP 2a inhibitors both by their modest dissociation constants and attenuated rate constants for acylation. A similar trend was noted for PBP 5 of Enterococcus faecium with some β-lactam antibiotics (24Hujer A.M. Kania M. Gerken T. Anderson V.E. Buynak J.D. Ge X. Caspers P. Page M.G. Rice L.B. Bonomo R.A. Antimicrob. Agents Chemother. 2005; 49: 612-618Crossref PubMed Scopus (26) Google Scholar).TABLE 1Kinetic parameters for interactions of cephalosporins (nitrocefin, cefepime, ceftazidime, and antibiotics 1–3) with PBP 2a in the absence and presence of compound 4Cephalosporin[4]k2Ksk2/Ksk3mms-1 × 103μmm-1 s-1s-1 × 106CefepimeaThe kinetic values for cefepime and ceftazidime (6) have been reported (6); these parameters are being given here for comparison with those of cephalosporins 1-3.01.5 ± 0.11620 ± 1450.9 ± 0.15.9 ± 0.5CeftazidimeaThe kinetic values for cefepime and ceftazidime (6) have been reported (6); these parameters are being given here for comparison with those of cephalosporins 1-3.01.0 ± 0.1670 ± 1151.5 ± 0.33.2 ± 0.2NitrocefinbThe kinetic values for nitrocefin have been reported (10); these parameters are being given here for comparison with those of cephalosporins 1-3.03.7 ± 0.3190 ± 2519 ± 37.2 ± 0.10.515 ± 2135 ± 12105 ± 1535 ± 102.035 ± 470 ± 14515 ± 10060 ± 4107 ± 132 ± 2205 ± 12—c—, no deacylation noted during 72 h of monitoring.0.514 ± 123 ± 1670 ± 30—2.040 ± 221 ± 11820 ± 110—205 ± 126 ± 1180 ± 20—0.516 ± 118 ± 1890 ± 26—2.038 ± 213 ± 13170 ± 135—304 ± 130 ± 2145 ± 8—0.519 ± 220 ± 1950 ± 90—2.050 ± 113 ± 13785 ± 260—a The kinetic values for cefepime and ceftazidime (6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar) have been reported (6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar); these parameters are being given here for comparison with those of cephalosporins 1-3.b The kinetic values for nitrocefin have been reported (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar); these parameters are being given here for comparison with those of cephalosporins 1-3.c —, no deacylation noted during 72 h of monitoring. Open table in a new tab In the discovery program for the three ABRI cephalosporins, compounds were screened for inhibition of PBP 2a in 96-well assays. This assay identifies molecules with enhanced ability to form the inhibited complex. Accordingly, we observe that the Ks values for these compounds are significantly lower than those of the other cephalosporins nitrocefin, cefepime, and ceftazidime. The corresponding values for 1, 2, and 3 are 32 ± 2, 26 ± 1, and 30 ± 2 μm (Table 1). Despite the substantially improved Ks values for cephalosporins 1–3, their respective k2/Ks parameters, the pseudo-second-order rate constants for the bimolecular encounter between PBP 2a and the cephalosporins, are merely 205 ± 12, 180 ± 20, and 145 ± 8 m–1 s–1, respectively. These values are smaller than we had expected for antibacterials that are effective in vivo (discussed further below). Two additional data are noteworthy. First, the k2 values for these compounds are comparable with those for the other cephalosporins (Table 1). Hence, their improved biological activity does not result from enhanced acylation of PBP 2a for the antibiotic by itself. Second, in contrast to the three other cephalosporins, we could not detect any deacylation of the ABRI cephalosporin acyl-enzyme species over 3 days of monitoring. Therefore, these cephalosporins acylate PBP 2a and give a complex that does not allow for the recovery of PBP 2a activity. This lack of deacylation may also contribute to the effectiveness of these antibiotics. Returning to the issue of the small values for k2/Ks, it is conceivable that the copy number of PBP 2a (∼800 molecules/S. aureus bacterium (25Pucci M.J. Dougherty T.J. J. Bacteriol. 2002; 184: 588-591Crossref PubMed Scopus (25) Google Scholar)) is so low that even these rather modest k2/Ks parameters are sufficient for inhibition of PBP 2a (and anti-MRSA activity). However, our earlier findings with a bacterial cell wall mimetic indicated that the active site opening of PBP 2a, from a closed to an open conformation, was enabled by the binding of the cell wall mimetic to an allosteric site outside of the transpeptidase active site (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). This possibility was considered for the ABRI cephalosporins. Compound 4 is an analogue of a fragment of the S. aureus cell wall, whose synthesis was reported recently by our laboratory (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). Compound 4 possesses the N-acetylglucosamine-N-acetylmuramic acid disaccharide backbone of the cell wall. To the N-acetylmuramic acid residue is appended the pentapeptide (N-acetylmuramic acid-l-Ala-γ-d-Gln-l-Lys-d-Ala-d-Ala) linker unit that is typical of S. aureus. The peptidoglycan of S. aureus also has a pentaglycyl moiety appended to the l-Lys side chain that participates in the PBP 2a-catalyzed cross-linking reaction. The omission of the pentaglycyl segment from the structure of 4 was deliberate, since we did not want 4 to be turned over by PBP 2a. The results for the ABRI cephalosporins with PBP 2a in the presence of compound 4 are intriguing (Table 1). As the concentration of the cell-wall analogue increased, the values for k2/Ks increased for each of antibiotics 1–3. The Kd (dissociation constant) for interaction of compound 4 outside the transpeptidase active site of PBP 2a is 1.2 ± 0.2 mm (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). We argued previously that the effective concentrations of the cell wall components that the PBPs experience on the bacterial cytoplasmic membrane are high (an entropic effect) (26Lee M. Hesek D. Suvorov M. Lee W. Vakulenko S. Mobashery S. J. Am. Chem. Soc. 2003; 125: 16322-16326Crossref PubMed Scopus (42) Google Scholar). However, it is likely that the Kd value for a larger fragment of peptidoglycan than compound 4 (i.e. a true polymeric cell wall) or for an analogue with the pentaglycyl moiety appended to the l-Lys residue would be lower (more favorable) than those measured for compound 4. Nonetheless, compound 4 is a useful surrogate for the bacterial cell wall, since it does bind to the requisite allosteric binding site outside of the active site with demonstrated saturation (10Fuda C. Hesek D. Lee M. Morio K. Nowak T. Mobashery S. J. Am. Chem. Soc. 2005; 127: 2056-2057Crossref PubMed Scopus (71) Google Scholar). The results with compound 4 indicate that PBP 2a is predisposed to enhanced inhibition by the ABRI cephalosporins in the presence of the cell wall. It is important to note that these effects are consistent and set trends for all three cephalosporins (Table 1). As the concentration of the cell wall fragment increases, the values of k2 increase (enhance acylation), and those of Ks decrease (more favorable binding). The absolute magnitude of these parameters is not as important (since compound 4 is a mere cell wall surrogate) as the consistent trends that they establish. Despite the fact that compound 4 is not a perfect substitute for the cell wall of S. aureus, the values measured for k2/Ks at concentrations of 4 that approach saturation (>Kd) of PBP 2a are within the range that should be significant in vivo (i.e. 2000–4000 m–1 s–1) (27Swinney D.C. Nat. Rev. Drug Discov. 2004; 3: 801-808Crossref PubMed Scopus (325) Google Scholar). We believe that these values should even be larger in the presence of a true polymeric cell wall, and they would contribute to the action of the novel cephalosporins in vivo. Our previous study described time-dependent changes in the CD spectrum of PBP 2a in the presence of oxacillin and ceftazidime, during the first few t½ values of the acylation process, documenting the existence of conformational changes during the acylation event. This was followed by the ultimate restoration to the CD spectrum of the mixture to that of the active enzyme, indicative of the completion of the deacylation event in due time (6Fuda C. Suvorov M. Vakulenko S.B. Mobashery S. J. Biol. Chem. 2004; 279: 40802-40806Abstract Full Text Full Text PDF PubMed Scopus (179) Google Scholar). A similar set of changes in the CD spectrum is observed for PBP 2a in the presence of cephalosporins 1–3 (Fig. 2), except the process for the onset is more rapid, and there is no recovery to the native state. The issue of lack of recovery of activity (and of the coincident altered CD spectrum) is addressed below. However, we were able to fit the data from the early parts of the CD spectra to a first-order process for the conformational change. In essence, the formation of the noncovalent complex of the antibiotic and PBP 2a sets off the conformational change that leads to acylation of the active site. The limitation of this method is the fact that the CD spectral acquisition is a relatively slow process, and we had as few as four data points for the extraction of the rate constants. As such, these rate constants should be considered approximations. However, it is interesting that the observed first-order rate constants from the CD measurements (kobs of 0.004, 0.002, and 0.001 s–1 for antibiotics 1, 2, and 3, respectively) are close to the corresponding values for the first-order acylation rate constants (k2, in the absence of compound 4; Table 1) of the respective antibiotics.FIGURE 2A, the far-UV circular dichroic measurement of PBP 2a in the presence of antibiotics. PBP 2a (3 μm, •), antibiotic 1 (6 μm, solid line), and PBP 2a (3 μm) with antibiotic 1 (6 μm) at 10 min (▴), 1 h (▪), 24 h (♦), 72 h (○). B, change in molar ellipticity of PBP 2a (3μm) at 208 (•) and 222 (○) as a function of time (200 min) for antibiotic 1 (6 μm). The inset shows the change in molar ellipticity of the PBP 2a (3 μm) at 208 (•) and 222 (○) over the longer duration (4320 min) for antibiotic 1 (6 μm). C, the far-UV circular dichroism spectrum of the PBP 2a (3μm, •), antibiotic 2 (6 μm, solid line), and PBP 2a (3 μm) with antibiotic 2 (6 μm) at 10 min (▴), 1 h (▪), 24 h (♦), 72 h (○). D, change in molar ellipticity of PBP 2a (3 μm) at 208 (•) and 222 (○) as a function of time for antibiotic 2. The inset shows the change in molar ellipticity of the PBP 2a (3 μm) at 208 (•) and 222 (○) over the longer duration (4320 min) for antibiotic 2 (6 μm). E, the far-UV circular dichroism spectrum of the PBP 2a (3 μm; •), antibiotic 3 (6 μm, solid line), and PBP 2a (3μm) with antibiotic 3 (6μm) at 10 min (▴), 1 h (▪), 24 h (♦), or 72 h (○). F, change in molar ellipticity of PBP2a (3 μm) at 208 (•) and 222 (○) as a function of time for antibiotic 3
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