Reticulocyte measurements as a bioassay for erythropoietin
1994; Elsevier BV; Volume: 12; Issue: 4 Linguagem: Inglês
10.1016/0731-7085(93)e0006-9
ISSN1873-264X
AutoresArminda G. Barbone, Beatriz Aparicio, David W. Anderson, Jaya Natarajan, David M. Ritchie,
Tópico(s)PARP inhibition in cancer therapy
ResumoThe possibility of using reticulocyte counts in peripheral blood to assay erythropoietic activity of recombinant human erythropoietin (r-HuEPO) was evaluated in normal mice. Mice were injected subcutaneously with r-HuEPO on days 0, 1 and 2 and bled on day 4 for reticulocyte count determinations, using an automated counting system with thiazole orange fluorescent staining and flow cytometric analysis. Reticulocyte counts increased in a dose-dependent fashion upon administration of r-HuEPO. The reticulocyte count was unaffected by asialylated EPO as well as other substances tested (interleukin-1, interleukin-3, dexamethasone, human chorionic gonadotropin). These data demonstrate the usefulness of employing reticulocyte counts as a rapid, specific and reproducible assay for in vivo erythropoietic activity of r-HuEPO.
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