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Comparison of an enzyme-linked immunosorbent assay (ELISA) with a radioimmunoassay (RIA) for the measurement of rat insulin

1990; Elsevier BV; Volume: 134; Issue: 1 Linguagem: Inglês

10.1016/0022-1759(90)90116-d

1872-7905

Helen V. Webster, Adrian J. Bone, Katherine A. Webster, Terence J. Wilkin,

Metabolism, Diabetes, and Cancer

A recently develop competitive enzyme-linked immunosorbent assay (ELISA) was compared with a conventional competitive radioimmunoassay (RIA) for the measurement of rat insulin in culture medium. Fifty-six samples were analysed by both assays. There was a correlation coefficient of r = 0.783 between results obtained using the two assay systems. The binding curves of the two assays were differently shaped, so that the ELISA gave good reproducibility over the concentration range 5–50 μU/ml insulin with inter and intra-assay coefficients of variation < 14%, but poor reproducibility at higher concentrations. Conversely, the RIA showed excellent reproducibility at concentrations greater than 50 μU/ml insulin, but poor sensitivity and high coefficients of variation below this level. The ELISA procedure offers practical advantages over the RIA, and performs well when measuring physiological concentrations of insulin.