Sensitive detection of pesticide using ENFET with enzymes immobilized by cross-linking and entrapment method
1999; Elsevier BV; Volume: 58; Issue: 1-3 Linguagem: Inglês
10.1016/s0925-4005(99)00103-3
ISSN1873-3077
AutoresK Wan, Jean‐Marc Chovelon, Nicole Jaffrézic‐Renault, А. П. Солдаткин,
Tópico(s)Electrochemical Analysis and Applications
ResumoTrichlorfon as a common pesticide is detected using a BuChE-FET sensor which is prepared either with cross-linked BSA–glutaraldehyde membrane or photocross-linkable PVA/SbQ (poly(vinyl alcohol) containing styrylpyridinium) membrane. On the basis of these BuChE-FET sensors, a biochemical test for trichlorfon in liquid was developed by enzyme inhibition. PVA/SbQ membrane shows better characteristics compared to BSA membrane: larger linear dynamic range in kinetic mode, better reproducibility, and above all, better stability in storage. Nevertheless, the both membranes present the same detection limit for trichlorfon: 10−6 M. The biosensor response after the enzyme inhibition can be partially recovered by using 2-methiodide pyridaldoxime, and the degree of enzymatic reactivation depends on the concentration of pesticides and on the time of incubation.
Referência(s)