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Effects of an alanyl-glutamine—based oral rehydration and nutrition therapy solution on electrolyte and water absorption in a rat model of secretory diarrhea induced by cholera toxin

2002; Elsevier BV; Volume: 18; Issue: 6 Linguagem: Inglês

10.1016/s0899-9007(02)00775-x

1873-1244

Aldo Â. M. Lima, G. H. P. Carvalho, Aline Almeida Figueiredo, Ângela R Gifoni, Alberto M. Soares, Eduardo A.T Silva, Richard L. Guerrant,

Electrolyte and hormonal disorders

Objectives: Recurring diarrhea and persistent diarrhea are commonly associated with malnutrition and long-term functional deficits. A beneficial approach would be to develop an alanyl-glutamine (AlaGln)—based oral rehydration and nutrition therapy (ORNT). We investigated the effect of an AlaGln-ORNT solution on electrolyte and water absorption in a rat model of secretory diarrhea induced by cholera toxin. Methods: Phenolsulfonphthalein (50 μg/mL) was used as a non-absorbable marker for calculation of net water and electrolyte transport. Solutions tested were Ringer’s solution, a glutamine-based ORNT (Gln-ORNT) solution, and an AlaGln-ORNT solution. Cholera toxin (1 μg/mL) was injected into lumen of rat small intestinal segments and incubated for 18 h before the initiation of the perfusion. Results: Cholera toxin induced significant secretion of electrolyte and water in the control Ringer’s solution. AlaGln-ORNT and Gln-ORNT solutions reduced the sodium secretory effect of cholera toxin by 128% and 36%, respectively. The net water secretion also was reduced by 95% and 60%, respectively, with the AlaGln-ORNT and Gln-ORTN solutions. Conclusions: These results showed that AlaGln-ORNT solution can enhance water and electrolyte intestinal absorption even better than glutamine or glucose and thus provide a potential novel approach for ORNT to break the vicious cycle of diarrhea and malnutrition. Clinical trials are now needed in children and adults with diarrhea and malnutrition.