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V H gene family utilization in different B‐cell lymphoma subgroups

1999; Wiley; Volume: 62; Issue: 2 Linguagem: Inglês

10.1111/j.1600-0609.1999.tb01732.x

1600-0609

Richard Rosenquist, Anita Lindström, Dan Holmberg, Jack Lindh, Göran Roos,

Lymphoma Diagnosis and Treatment

Abstract: V H gene family specific polymerase chain reaction (PCR) amplification was performed in 87 B‐cell lymphoma samples from 4 different subgroups. No apparent restriction in the V H gene usage was found in follicular lymphomas, lymphoplasmacytoid lymphomas or large B‐cell lymphomas, whereas a biased V h 1 utilization was shown in patients with chronic lymphocytic leukemia. Eleven of 18 chronic lymphocytic leukemia cases utilized the V H 1 gene family, and nucleotide sequencing of the V H 1 gene rearrangements revealed that a majority utilized the DP10 (51p1) germline gene, which has been reported to be strongly associated with autoimmune disease. No V H 5 or V H 6 rearrangements were amplified in the chronic lymphocytic leukemia subgroup, 2 gene families which previously have been found to be over‐represented in these patients. In a high proportion (40%) of large B‐cell lymphomas, V H gene family‐specific PCR failed to amplify any rearrangement. Using primers hybridizing to the framework regions 2 and 3 and Southern blot analysis of the immunoglobulin heavy chain locus, clonal rearrangements were displayed in two‐thirds of these PCR negative cases. However, the rearrangement status could not be elucidated in 5 of 35 patients with large B‐cell lymphoma.