Cationic liposome mediated transgene expression in the guinea pig cochlea
1999; Elsevier BV; Volume: 128; Issue: 1-2 Linguagem: Inglês
10.1016/s0378-5955(98)00196-8
ISSN1878-5891
AutoresMichael J. Wareing, Anand N. Mhatre, Robert Pettis, Jay J. Han, Terry Haut, Markus HF Pfister, Keelung Hong, Wei Wen Zheng, Anil K. Lalwani,
Tópico(s)Virus-based gene therapy research
ResumoSensorineural hearing loss affects nearly 10% of the American population that is refractory to conventional therapy. Gene therapy represents an intervention with potential therapeutic efficacy. We studied the feasibility of cationic liposome mediated gene transfer within the guinea pig cochlea in vivo following direct microinjection into the cochlea. Transgene expression was persistent up to 14 days in the neurosensory epithelia and surrounding tissue without toxicity and inflammation in the target organ. This study represents the first successful use of cationic liposomes for cochlear gene transfer thus providing a safe and rapid alternative to the use of recombinant viral vectors in gene therapy for inner ear disorders.
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