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HLA‐DR and ‐DQ polymorphism in Cameroon

2001; Wiley; Volume: 58; Issue: 1 Linguagem: Inglês

10.1034/j.1399-0039.2001.580101.x

1399-0039

Nattiya Pimtanothai, Carolyn Katovich Hurley, Rose G. F. Leke, William Klitz, A.H. Johnson,

Immune Cell Function and Interaction

Abstract: HLA‐DRB1, ‐DQA1 and ‐DQB1 allele frequencies were determined by high‐resolution polymerase chain reaction using sequence‐specific oligonucleotide probes (PCR‐SSOP) and/or DNA sequencing in 126 healthy individuals in Cameroon. Eighteen DRB1, 11 DQA1, and 18 DQB1 alleles were observed. The most common alleles at each locus were DRB1*1503 (29%), DRB1*1301 (13%); DQA1*0102 (38%), DQA1*0302 (11%), DQA1*0401 (11%); and DQB1*0602 (31%), DQB1*0301 (13%), DQB1*0501 (12%). Forty‐four different haplotypes were identified including 12 novel haplotypes demonstrating the HLA class II diversity resulting from allele combinations in this population. A single predominant DRB1*1503‐DQA1*0102‐DQB1*0602 haplotype was observed with a frequency of 27%. In summary, this study of HLA class II polymorphism in Cameroon demonstrates the extent of diversity in this population.