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BIBTEX RIS

Identification of genes involved in DNA replication of the Autographa californica baculovirus.

1994; National Academy of Sciences; Volume: 91; Issue: 23 Linguagem: Inglês

10.1073/pnas.91.23.11212

ISSN

1091-6490

Autores

Marcel Kool, Christian H. Ahrens, Rob Goldbach, George F Rohrmann, Just M. Vlak,

Tópico(s)

Transgenic Plants and Applications

Resumo

By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was identified in the carboxyl-terminal region of IE-1.

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