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The labelling of mammalian spermatozoa with radioactive tracers

1958; Wiley; Volume: 137; Issue: 3 Linguagem: Inglês

10.1002/jez.1401370302

1097-010X

J.L. Sirlin, Robert G. Edwards,

Genetically Modified Organisms Research

Journal of Experimental ZoologyVolume 137, Issue 3 p. 363-388 Article The labelling of mammalian spermatozoa with radioactive tracers J. L. Sirlin, J. L. Sirlin Institute of Animal Genetics, University of Edinburgh Research worker of the British Empire Cancer Campaign.Search for more papers by this authorR. G. Edwards, R. G. Edwards Institute of Animal Genetics, University of Edinburgh Working on a research grant from the Agricultural Research Council.Search for more papers by this author J. L. Sirlin, J. L. Sirlin Institute of Animal Genetics, University of Edinburgh Research worker of the British Empire Cancer Campaign.Search for more papers by this authorR. G. Edwards, R. G. Edwards Institute of Animal Genetics, University of Edinburgh Working on a research grant from the Agricultural Research Council.Search for more papers by this author First published: April 1958 https://doi.org/10.1002/jez.1401370302Citations: 12 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Literature Cited Alfert, M. 1956 Chemical differentiation of nuclear proteins during spermatogenesis in the salmon. J. Biophys. Biochem. Cytol., 2: 109–114. Auerbach, C., and B. M. Slizynski 1956 Sensitivity of the mouse testis to the mutagenic action of x-rays. Nature, 177: 376–377. Bateman, A. J. 1956–57 Sensitivity of immature mouse sperm to the mutagenic effects of x-rays. Nature, 178: 1278–1280; Nature, 179: 727. Barghava, P. M. 1957 Incorporation of radioactive amino-acids in the proteins of bull spermatozoa. Nature, 179: 1120–1121. Bishop, D. W. 1952 The metabolic machinery of sperm activity: In Studies on Testis and Ovary, Eggs and Sperm. E. T. Engle, Ed. Charles C Thomas, Springfield, Illinois. Boyd, G. A., G. W., Casarett, K. I. Altman, T. R. Noonan and K. Salomon 1948 Autoradiographs of C14 incorporated in individual blood cells. Science, 108: 529–531. Brenner, S. 1957 On the impossibility of all overlapping triplet codes in information transfer from nucleic acid to proteins. Proc. Nat. Acad. Sc., 43: 687–694. Crick, F. H. C., J. S., Griffith and L. E. Orgel 1957 Codes without commas. Proc. Nat. Acad. Sc., 43: 416–421. Dallam, R. D., and L. E. Thomas 1953 Chemical studies on mammalian spermatozoa. Biochem. Biophys. Acta, 11: 79–89. Daly, M. M., A. E., Mirsky and H. Ris 1951 The amino acid composition and some properties of histones. J. Gen. Phys., 34: 439–450. Daoust, R., and Y. Clermont 1955 Distribution of nucleic acids in germ cells during the cycle of the seminiferous epithelium in the rat. Am. J. Anat., 96: 255–283. Di Stefano, H. S., and D. Mazia 1952 The role of the mid-piece in the incorporation of phosphorus-32 into RNA of Arbacia spermatozoa (abs.). Biol. Bull., 103: 299. Edwards, R. G., and J. L. Sirlin 1956 Labelled pronuclei in mouse eggs fertilized by labelled sperm. Nature, 177: 429. Edwards, R. G., and J. L. Sirlin 1957 Studies in gametogenesis, fertilization, and early development in the mouse, using radioactive tracers. Internat. J. Fertility, 2: 185–199. Edwards, R. G., and J. L. Sirlin 1958 Radioactive tracers and fertilization in mammals. Endeavour, 17: 65, 42–47 (1958). Edwards, R. G., and J. L. Sirlin Effect of 200 r of x-rays on the rate of spermatogenesis and spermiogenesis in the mouse. Exp. Cell Res. (in press). Feughelman, M., R., Langridge, W. E. Seeds, A. R. Stokes, H. R. Wilson, C. W. Hooper, M. H. F. Wilkins, R. K. Barclay and L. D. Hamilton 1955 Molecular structure of deoxyribose nucleic acid and nucleoprotein. Nature, 175: 834–838. Flipse, R. J., and A. A. Benson 1957 Catabolism of glycine-C14 by washed bovine spermatozoa. Exp. Cell Res., 13: 611–614. Glucksmann, A., A., Howard and S. R. Pelc 1955 Incorporation of S35-DL-methionine in mouse tissues as indicated by autoradiographs. J. Anat., 89: 13–18. Heath, H., C., Rimington, T. Glover, T. Mann and E. Leone 1953 Studies using radioactive sulphur on ergothioneine formation in the pig. Biochem. J., 54: 606–611. Hertwig, P. 1938 Unterschiede in der Entwicklungsfähigkeit von F1 Mäusen nach Röntgen-Bestrahlung von Spermatogonien, fertigen und unfertigen Spermatozoen. Biol. Zentralblatt, 58: 273–301. Howard, A., and S. R. Pelc 1950 P32 autoradiographs of mouse testis. Brit. J. Rad., 23: 634–641. Knobloch, A., and R. Vendrely 1956 An estimation of the nature of nucleoproteins by an analytical method. Nature, 178: 261–262. Lajtha, L. G., R., Oliver and F. Ellis 1954 Incorporation of P32 and adenine C14 into DNA by human bone marrow cells in vitro. Brit. J. Cancer, 8: 367–379. Lamerton, L. F., and E. B. Harriss 1953 Factors affecting resolution in autoradiography. Int. Conf. Sci. Appl. Photography, Lond. Lamerton, L. F., and E. B. Harriss 1954 Resolution and sensitivity considerations in autoradiography. J. Photog. Sci., 42: 135–144. Lasnitzki, I., R. E. F., Matthews and J. D. Smith 1954 Incorporation of 8-azaguanine into nucleic acids. Nature, 173: 346–348. Leuchtenberger, C., I., Murmanis, L. Murmanis, S. Ito and D. R. Weir 1956 Interferometric dry mass and microspectophotometric arginine determinations on bull sperm nuclei with normal and abnormal DNA content. Chromosoma, 8: 73–86. Lorenz, F. W., M., Cavoulas and J. D. Carson 1950 Deposition of phosphorus-32 in cock spermatozoa (abs.). Poultry Sci., 29: 769. MacMillan, E. W. 1957 Report by G. I. M. Swyer in: Studies on Fertility. Nature, 180: 226–227. Malkin, H. M. 1953 Incorporation of glycine-2-C14 and adenine-4,6-C14 into DNA of mature sea urchin sperm. Biochem. Biophys. Acta, 12: 585–586. Mann, T. 1954 The Biochemistry of Semen. Methuen, London. Marrian, D. H., V. L., Spicer, M. E. Balis and G. B. Brown 1951 Purine incorporation into pentose nucleotides of the rat. J. Biol. Chem., 189: 533–541. Matthews, R. E. F. 1957 The biological effects of 8-azapurines. In: Chemistry and Biology of Purines. Ciba Foundation Symposium. Churchill, London, pp. 270–282. Mellors, R. C., and J. Hlinka 1955 Quantitative cytology and cytopathology. IV. Interferometric measurement of the anhydrous organic mass (dry weight) of genetic material in sperm nuclei of the mouse, the rat and the guinea pig. Exp. Cell Res., 9: 128–134. MOUSE NEWS LETTEE 1957 No. 17, 38 (July). Muller, H. J. 1954 The manner of production of mutations by radiation. In: Radiation Biology, vol. I. Ed. A. Hollaender. McGraw-Hill Co., N. Y., pp. 475–626. Oakberg, E. F. 1956a A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am. J. Anat., 99: 391–413. Oakberg, E. F. 1956b Duration of spermatogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium. Am. J. Anat., 99: 507–516. Oakberg, E. F. 1957 Duration of spermatogenesis in the mouse. Nature, 180: 1137–1139. Oftedal, P., and J. Mossige 1957 Incorporation of phosphorus-32 and mutagenesis during spermatogenesis in Drosophila. Nature, 179: 104–105. Ortavant, R. 1956 Autoradiographie des cellules germinales du testicule de bélier. Dureé des phénoménes spermatogénétiques. Arch. Anat. mic. Morph. exp., 45: 1–10. Pelc, S. R. 1957 On the connection between the synthesis of RNA and DNA in the testis of the mouse. Exp. Cell Res., 12: 320–324. Pelc, S. R., and A. Howard 1956 A difference between spermatogonia and somatic tissues of mice in the incorporation of (8-C14)-adenine into deoxyribonucleic acid. Exp. Cell Res., 11: 128–134. Risley, P. A. 1955 Autoradiographs of hamster spermatozoa. J. Cell. and Comp. Physiol., 45: 21–32. Sirlin, J. L., and R. G. Edwards 1955 The labelling of mouse sperm by adenine8-C14. Exp. Cell Res., 9: 596–599. Sirlin, J. L., and R. G. Edwards 1957a Sensitivity of immature mouse sperm to the mutagenic effects of x-rays. Nature, 179: 725–727. Sirlin, J. L., and R. G. Edwards 1957b Duration of spermatogenesis in the mouse. Nature, 180: 1137–1139. Smellie, R. M. S., and J. N. Davidson 1956 The incorporation of carbonlabelled precursors into the nucleic acids of various tissues in the intact rabbit. Experimentia, 12: 422–424. Swift, H. H. 1950 The desoxyribose nucleic acid content of animal nuclei. Phys. Zool., 23: 169–198. Taylor, J. H. 1955 Autoradiography at the cellular level. In: Physical Techniques in Biological Research, vol. 3. Eds. G. Oster and A. W. Pollister. Academic Press, N. Y., pp. 545–576. Watson, J. D., and F. H. C. Crick 1953 The structure of DNA. Symp. Quant. Biol., 18: 123–131. Yankwick, P. E., and J. W. Weigl 1948 The relation of backscattering to self-absorption in routine beta-ray measurements. Science, 107: 651–653. Citing Literature Volume137, Issue3April 1958Pages 363-388 ReferencesRelatedInformation