Urine and faecal porphyrin profiles by reversed-phase high-performance liquid chromatography in the porphyrias
1984; Elsevier BV; Volume: 139; Issue: 1 Linguagem: Inglês
10.1016/0009-8981(84)90192-x
ISSN1873-3492
Autores Tópico(s)Heme Oxygenase-1 and Carbon Monoxide
ResumoA novel reversed-phase system is described for the simultaneous separation of type I and type III isomers of uro-, heptacarboxylic-, hexacarboxylic-, pentacarboxylic-and copro-porphyrins and the dicarboxylic meso- and proto-porphyrins. The porphyrins were resolved on a Hypersil-SAS column eluted with 10% (v/v) acetonitrile in 1 mol/1 ammonium acetate, pH 5.16 (solvent A) and 10% (v/v) acetonitrile in methanol (solvent B) with a linear gradient from 100% A (0%B) to 35% A (65% B) in 30 min followed by isocratic elution at 65% B for a further 10 min. The method is simple and reproducible and has been applied to the analysis of porphyrins in urine and faeces from patients with acute intermittent, variegate, hereditary copro, congenital erythropoietic, erythrohepatic proto and symptomatic porphyrias.
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