Identification of a perinuclear positioning element in human subtelomeres that requires A-type lamins and CTCF
2009; Springer Nature; Volume: 28; Issue: 16 Linguagem: Inglês
10.1038/emboj.2009.201
ISSN1460-2075
AutoresAlexandre Ottaviani, Caroline Schluth‐Bolard, Sylvie Rival‐Gervier, Amina Boussouar, Delphine Rondier, Andrea M. Foerster, Julia Morere, Serge Bauwens, Sophie Gazzo, Evelyne Callet‐Bauchu, Éric Gilson, Frédérique Magdinier,
Tópico(s)Nuclear Structure and Function
ResumoArticle30 July 2009free access Identification of a perinuclear positioning element in human subtelomeres that requires A-type lamins and CTCF Alexandre Ottaviani Alexandre Ottaviani Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, FrancePresent address: Erasmus Medical Center, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands Search for more papers by this author Caroline Schluth-Bolard Caroline Schluth-Bolard Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Sylvie Rival-Gervier Sylvie Rival-Gervier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France INRA, ENVA, UMR 1198, Biologie du développement et de la Reproduction, CNRS FRE 2857, Jouy-en-Josas Search for more papers by this author Amina Boussouar Amina Boussouar Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Delphine Rondier Delphine Rondier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Andrea M Foerster Andrea M Foerster Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, FrancePresent address: Gregor Mendel-Institute of Molecular Plant Biology, Dr Bohr-Gasse 3, 1030 Vienna, Austria Search for more papers by this author Julia Morere Julia Morere Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Serge Bauwens Serge Bauwens Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Sophie Gazzo Sophie Gazzo Service d'Hématologie Biologique, Centre Hospitalier Lyon Sud—Hospices Civils de Lyon et Université Claude Bernard Lyon I, EA, Pierre-Bénite, Cedex, France Search for more papers by this author Evelyne Callet-Bauchu Evelyne Callet-Bauchu Service d'Hématologie Biologique, Centre Hospitalier Lyon Sud—Hospices Civils de Lyon et Université Claude Bernard Lyon I, EA, Pierre-Bénite, Cedex, France Search for more papers by this author Eric Gilson Corresponding Author Eric Gilson Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Frédérique Magdinier Corresponding Author Frédérique Magdinier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Alexandre Ottaviani Alexandre Ottaviani Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, FrancePresent address: Erasmus Medical Center, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands Search for more papers by this author Caroline Schluth-Bolard Caroline Schluth-Bolard Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Sylvie Rival-Gervier Sylvie Rival-Gervier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France INRA, ENVA, UMR 1198, Biologie du développement et de la Reproduction, CNRS FRE 2857, Jouy-en-Josas Search for more papers by this author Amina Boussouar Amina Boussouar Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Delphine Rondier Delphine Rondier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Andrea M Foerster Andrea M Foerster Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, FrancePresent address: Gregor Mendel-Institute of Molecular Plant Biology, Dr Bohr-Gasse 3, 1030 Vienna, Austria Search for more papers by this author Julia Morere Julia Morere Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Serge Bauwens Serge Bauwens Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Sophie Gazzo Sophie Gazzo Service d'Hématologie Biologique, Centre Hospitalier Lyon Sud—Hospices Civils de Lyon et Université Claude Bernard Lyon I, EA, Pierre-Bénite, Cedex, France Search for more papers by this author Evelyne Callet-Bauchu Evelyne Callet-Bauchu Service d'Hématologie Biologique, Centre Hospitalier Lyon Sud—Hospices Civils de Lyon et Université Claude Bernard Lyon I, EA, Pierre-Bénite, Cedex, France Search for more papers by this author Eric Gilson Corresponding Author Eric Gilson Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Frédérique Magdinier Corresponding Author Frédérique Magdinier Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France Search for more papers by this author Author Information Alexandre Ottaviani1, Caroline Schluth-Bolard1, Sylvie Rival-Gervier1,2, Amina Boussouar1, Delphine Rondier1, Andrea M Foerster1, Julia Morere1, Serge Bauwens1, Sophie Gazzo3, Evelyne Callet-Bauchu3, Eric Gilson 1 and Frédérique Magdinier 1 1Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, Lyon Cedex, France 2INRA, ENVA, UMR 1198, Biologie du développement et de la Reproduction, CNRS FRE 2857, Jouy-en-Josas 3Service d'Hématologie Biologique, Centre Hospitalier Lyon Sud—Hospices Civils de Lyon et Université Claude Bernard Lyon I, EA, Pierre-Bénite, Cedex, France *Corresponding authors. Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS UMR 5239, UCBL1, IFR128, 46 allée d'Italie, 69364 Lyon Cedex 07, France. Tel.:+33 4 7272 8453; Fax:+33 4 7272 8080; E-mail: [email protected] or Tel.:+33 4 7272 8663; E-mail: [email protected] The EMBO Journal (2009)28:2428-2436https://doi.org/10.1038/emboj.2009.201 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The localization of genes within the nuclear space is of paramount importance for proper genome functions. However, very little is known on the cis-acting elements determining subnuclear positioning of chromosome segments. We show here that the D4Z4 human subtelomeric repeat localizes a telomere at the nuclear periphery. This perinuclear activity lies within an 80 bp sequence included within a region known to interact with CTCF and A-type Lamins. We further show that a reduced level of either CTCF or A-type Lamins suppresses the perinuclear activities of D4Z4 and that an array of multimerized D4Z4 sequence, which has lost its ability to bind CTCF and A-type Lamins, is not localized at the periphery. Overall, these findings reveal the existence of an 80 bp D4Z4 sequence that is sufficient to position an adjacent telomere to the nuclear periphery in a CTCF and A-type lamins-dependent manner. Strikingly, this sequence includes a 30 bp GA-rich motif, which binds CTCF and is present at several locations in the human genome. Introduction The spatial distribution of chromosomal domains within the nucleoplasm has an important function in the regulation of eukaryotic genomes (Csink and Henikoff, 1996; Maillet et al, 1996; Brown et al, 1997; Andrulis et al, 1998; Taddei et al, 2006; Nagai et al, 2008). Very little is known on the cis-acting elements governing the subnuclear localization of genes or groups of genes, especially in mammals. However, these elements are expected to contribute to accurate genetic and epigenetic regulations. As examples, the localization of chromosome regions at the periphery of the nucleus seems to have a central function in gene regulation and DNA repair (Therizols et al, 2006; Finlan et al, 2008; Kumaran and Spector, 2008; Reddy et al, 2008). Nevertheless, the precise regulation of genes located at the nuclear rim remains poorly understood as the expression of some genes appears unaffected by their proximity to the nuclear periphery (Finlan et al, 2008; Kumaran and Spector, 2008). In metazoan, the lamina, which coats the inner surface of the nuclear envelope, has an important function in the perinuclear localization of chromosome segments. For instance, in mice, lamin B1 is involved in the perinuclear localization of chromosome 18 (Malhas et al, 2007) and recent large-scale mappings of chromosomal domains attached to B-type lamins performed in Drosophila and human cells, defined the Lamin-associated domains or LADs (Pickersgill et al, 2006; Guelen et al, 2008), which correlate with silenced regions. In human cells, some of the LADs are flanked by binding sites for CTCF, suggesting that the insulator protein allows a functional partitioning of the human genome by separating active and inactive domains. The telomeres are not randomly localized within the nucleoplasm and can be found at the nuclear periphery (Gilson et al, 1993). This positioning varies greatly among organisms, cell types, cell cycle stages and individual telomeres. At the bouquet stage for instance, the clustering of all telomeres at the edge of the nucleus, is a nearly universal feature of meiosis (Scherthan, 2007). In budding yeast, the 32 telomeres gather into 4–6 foci, associated with the nuclear envelope (Gotta et al, 1996) that sequesters heterochromatic factors (Gotta et al, 1996; Maillet et al, 1996). The peripheral positioning of yeast telomere is mediated by at least two complexes bound to the telomeric chromatin, suggesting that, in this model organism, the telomere by itself behaves as a perinuclear positioning element (Taddei et al, 2005). Consistent with this hypothesis, de novo telomeres formed in yeast, devoid of natural subtelomeric repeats adopt a perinuclear localization (Tham et al, 2001). A perinuclear positioning of telomeres is also observed in Plasmodium, where it favours subtelomeric gene conversion (Freitas-Junior et al, 2000), whereas in plants, telomeres are observed either close to the nuclear periphery (Rawlins and Shaw, 1990) or around the nucleolus (Fransz et al, 2002). In mammalian nuclei, telomeres adopt different locations (Luderus et al, 1996). Although human telomeres are clustered at the nuclear periphery in sperm (Zalenskaya et al, 2000), most telomeres in lymphocyte nuclei are located in the interior of the nucleoplasm (Weierich et al, 2003). Thus, it seems that by default, human telomeres are localized internally in most cell types. However, some subtelomeric elements may antagonize this internal localization and target their proximal telomere to the nuclear envelope as suggested by the presence of LADs at different subtelomeres (Guelen et al, 2008). Such an example of localization at the periphery of the nucleus is the positioning of the 4q35 subtelomeric locus, involved in the facio-scapulo-humeral dystrophy (FSHD) (Masny et al, 2004; Tam et al, 2004). The existence in metazoan of cis-acting DNA elements targeting chromosomal regions to the nuclear envelope remains elusive and a key question regarding the function of perinuclear localization is to know whether it is the cause or consequence of differential gene expression or genomic nature. To identify and characterize such elements, we investigated the ability of D4Z4, a 3.3 kb macrosatellite repeat present at the 4q35 locus (Hewitt et al, 1994; Lyle et al, 1995), to direct the localization of an associated telomere towards nuclear periphery. D4Z4 is repeated in tandem at several chromosomal loci including the short arm of acrocentric chromosomes, the pericentric region of chromosome 1q and the telomeric regions of the long arms of chromosomes 4 and 10 (Hewitt et al, 1994; Lyle et al, 1995). Normal 4q35 ends carry from 11 up to 150 copies of this element, whereas this number is reduced to 1–10 repeats in patients affected with the autosomal dominant FSHD (van Deutekom et al, 1993; Winokur et al, 1994). We report here that D4Z4, which we previously characterized as a CTCF- and A-type Lamins-dependent insulator (Ottaviani et al, 2009), behaves as a potent perinuclear cis-acting positioning element. Impressively, this property depends on CTCF and A-type Lamins binding and is lost upon multimerization of the repeats, uncovering a striking link between perinuclear localization, CTCF and A-type Lamins and transcriptional insulation. Results and discussion D4Z4 behaves as a perinuclear positioning element To evaluate the putative role of D4Z4 in the subnuclear localization of telomeres, we artificially tagged different telomeres by transfecting human cells with DNA molecules carrying D4Z4 sequences inserted between a seed of (TTAGGG)n telomeric repeats and a hygromycin-eGFP cassette (Figure 1A and B). Telomere fragmentation is based on the non-targeted introduction of cloned telomeres into mammalian cells and de novo telomere formation after telomeric healing (Farr et al, 1992). Successful de novo formation of hygromycin-tagged telomeres was confirmed in individual clones by detection of a diffuse hybridization signal in Southern blot or in the population of transfected cells by fluorescence in situ hybridization (FISH) of metaphase spreads (data not shown and Figure 1A). In agreement with earlier data (Koering et al, 2002), the rate of de novo telomere formation in stably transfected C33A cells is very high reaching 85–94% of the hygromycin-resistant cells (Supplementary Table S1). Therefore, polyclonal populations of stably transfected cells are representative of pools of independent clones of hygromycin-tagged telomeres and further experiments were performed on populations. Figure 1.D4Z4 relocates eGFP-tagged telomeres towards the nuclear periphery. (A) The constructs are derived from the pCMV vector, which carries a Hygromycin resistance gene fused to the herpes simplex virus type 1 thymidine kinase suicide gene (HyTK) and an eGFP reporter gene both driven by CMV promoters, and carry a telomere seed (depicted by arrows) that allows telomeric fragmentation. We inserted different sequences between the reporter and the telomere to investigate their respective effect on telomere positioning. Successful de novo formation of eGFP-tagged telomeres or internal integration of the CMV construct were confirmed by fluorescence in situ hybridization (FISH) on metaphase spreads (photographs 1, 2, respectively, Supplementary Table S1). M-FISH analysis performed on cells containing the pCMV Telo vector (T) or a vector with D4Z4 (T1X) confirmed that constructs carrying the D4Z4 sequence do not integrate preferentially at certain chromosomes (Supplementary Figure S2). (B) CMV: empty vector; T: empty telomeric vector; T1X: insertion of a 3.3 kb D4Z4 element between eGFP and the telomere in T; T-5′ HS4: insertion of the 1.2 kb chicken β-globin insulator (Chung et al, 1993). Schematic representation of the normal 4q35 allele. (C) Confocal section of T, T1X cells stained with an eGFP probe (single red dot) or endogenous 4q with a 4qtel probe staining both alleles (two red dots). Representation of the analysis of a nucleus after two-colour 3D-FISH (right panel). We considered the outer limit of the Lamin B signal (blue) as the edge of the nucleus (100%). Distribution of the FISH signals within the nuclear volume was calculated from the centre (0%) to the outer edge of the sphere after reduction of the outer signal (VL=nuclear volume=100%) until it overlaps with the FISH signal (Vl=x% of VL) (Supplementary Figure S1). Experiments were performed on three to four independent populations of cells (Supplementary Table S1). (D) Histogram displaying the mean positioning of natural and fragmented telomeres as their mean values of volume ratio (Vl/VL)±s.d. shown by error bars (y-axis). Data sets were compared with the Kruskal–Wallis statistic test (P<2.4 × 10−9, n=number of interphase nuclei). Brackets identify two groups where all conditions are significantly different from the other group, based on FDR determination. (E) Distribution of the FISH signal from the centre to the outer rim of the nucleus. Lamin B signal occupies the outermost 18% of the nuclear radius considered (grey shadow). Download figure Download PowerPoint The tri-dimensional distribution of the FISH signal corresponding to the hygromycin-eGFP cassette was determined in different cell populations as a function of the nuclear volume after delineation of the nuclear periphery by simultaneous immunolocalization of B-type Lamins (Figure 1C; Supplementary Figure S1). Using this 3D-immuno-FISH method, we showed that de novo formed telomeres, devoid of natural subtelomeric sequences, have the tendency to localize in the innermost nuclear volume (T construct in Figure 1C–E). By contrast, the eGFP-hygromycin construct but devoid of telomere seed appears randomly distributed between the interior and the periphery of the nucleoplasm (Figure 1D and E). These results are in agreement with several reports suggesting that human telomeres have the intrinsic property to be anchored to internal sites (Luderus et al, 1996; Weierich et al, 2003; Tam et al, 2004). As expected from earlier observations, the 4q35 locus is enriched in a peripheral zone of the nucleus (Figure 1C–E) (Masny et al, 2004; Tam et al, 2004). Strikingly, telomeres associated to a single copy of D4Z4 have a more peripheral distribution (T1X construct) than D4Z4-less telomeres (T construct) (Figure 1C–E), suggesting that this element might be specialized in the subnuclear positioning of telomeres. The perinuclear localization of D4Z4-associated telomeres unlikely results from biases in telomere seeding or in chromosome choice as we observed a similar rate of telomere formation (Supplementary Table S1) and no preferential insertion between populations of cells transfected with seeding constructs with or without D4Z4 (Supplementary Figure S2). We recently showed that D4Z4 act as a CTCF-dependent insulator protecting from position effect variegation and enhancer-promoter communication (Ottaviani et al, 2009). The protection against silencing is mediated in part by CTCF and A-type Lamins, which bind in vivo to D4Z4 as shown by chromatin immunoprecipitation. Upon multimerization of D4Z4, both CTCF binding and insulator activity are lost suggesting that FSHD is associated with a gain of function of CTCF (Ottaviani et al, 2009). Interestingly, insulators are often anchored at fixed nuclear substructures including the nuclear periphery in eukaryotes (Gerasimova and Corces, 2001; Gaszner and Felsenfeld, 2006; Valenzuela and Kamakaka, 2006). Therefore, to test whether peripheral tethering of telomeres is a common property of CTCF-dependent vertebrate insulators, we compared D4Z4 with the 5′ HS4 chicken β-globin CTCF-dependent insulator inserted near telomeres (Figure 1B). We showed that 5′HS4-tagged telomeres are localized in the innermost nuclear volume as observed for the T construct, suggesting that the peripheral positioning activity is an intrinsic property of D4Z4 (Figure 1C–E). We have previously identified a shorter fragment with anti-silencing activity within D4Z4 and we mapped by deletion the region of D4Z4 involved in this perinuclear positioning activity. We found three truncated forms of D4Z4 that recapitulate both the insulator activity and the peripheral localization of telomeres of the full-length repeat (Figure 2, T1XΔB1, T1XΔB1-3, T1XΔE), whereas the very proximal 382 bp at the 5′ end of D4Z4 (Figure 2, T1XΔB2-3) harbours the same distribution than a D4Z4-less telomere. Impressively, a short sequence of 80 bp that harbours anti-silencing activity (Supplementary Figure S3) is sufficient to localize a telomere closer to the periphery (Figure 2, Tld). Therefore, we conclude that D4Z4 has the ability to direct a subtelomeric region at or near the nuclear envelope and that this property is mediated, at least in part, by a segment present at the 5′ end of the D4Z4 repeat. Figure 2.Identification of the tethering element within D4Z4. (A) Schematic representation of this element from position 1–3303 relative to the two flanking KpnI sites (K) (to scale), the different regions within D4Z4 are shown. Each repeat contains two classes of repetitive DNA, LSau, a repetitive element associated with heterochromatin regions, a GC-rich low copy repeat, hhspm3 displaying sperm-specific DNA hypomethylation and a region named region A. DUX4 corresponds to an ORF with a double homeobox encoding the DUX4 protein putatively involved in the disease. The position of the CTCF site is shown (Ottaviani et al, 2009). The restriction sites used for the cloning of D4Z4 subfragments are indicated (B: BamHI; Bl: BlpI; E: EheI) and the different constructs used are depicted (B). (C) Subnuclear positioning of eGFP-tagged telomeres in cells containing different fragments of D4Z4. The number of nuclei analysed per construct is indicated (n) together with the P-values determined with the Mann–Whitney tests using the T construct as the reference. Significant conditions after false discovery rate (FDR, α=0.05) correction for multiple comparisons are marked by asterisk. These results reveal that constructs containing the proximal insulator of D4Z4 displace a telomere towards the nuclear periphery, whereas T1XΔ2-3 occupies the same positioning as the T constructs. Download figure Download PowerPoint CTCF and A-Lamins contribute to the localization of D4Z4 at the nuclear periphery As the different fragments involved in the perinuclear positioning possess an anti-silencing activity mediated in vivo by CTCF and A-type Lamins (Ottaviani et al, 2009; Supplementary Figure S3), we wondered whether these proteins are involved in the positioning activity of D4Z4 as well. When the expression of CTCF or LMNA is significantly reduced by RNA interference (Supplementary Figure S4), the distribution of D4Z4-tagged telomeres is more internal (Figure 3A and B) revealing an essential role for these proteins in the perinuclear distribution of D4Z4. Importantly, the effect of CTCF depletion is specific for D4Z4 as our RNA interference conditions are (i) not accompanied by a modification of the localization of D4Z4-less telomeres (ii) nor by any global disturbance of the nuclear architecture as shown by the distribution of the 10q locus and X chromosome (Figure 3A, C and D) and (iii) the CTCF-dependent 5′HS4 insulator of the chicken β-globin locus artificially integrated at chromosome ends (T-5′ HS4) remains in the inner nuclear space (data not shown). We conclude that D4Z4 positions its associated seeded telomere to the nuclear periphery through an interaction with CTCF and A-type Lamins, whereas in the absence of CTCF, the wild-type 4q35 locus remains at the nuclear periphery (Figure 3A), suggesting additional pathways for the anchoring of this region. Figure 3.CTCF and A-type Lamins contribute to the positioning of D4Z4-tagged telomeres. (A) Cells containing the T1X or the T transgene transiently transfected with CTCF (KD CTCF) or negative (mock) siRNA were compared using the Mann–Whitney test. Depletion of CTCF correlates with the relocation of D4Z4-tagged telomere to the interior of the nucleus (P=0.0198) whereas the T construct (P=0.597) and the 4q (P=0.536) are not affected. (B) LMNA depletion significantly displaces the T1X construct (P=0.0376) but does not affect the distribution of the 4q telomere (P=0.6973) (Mann–Whitney). (C) To ascertain the global integrity of the nucleus architecture after the transient knock-down of CTCF, we analysed the distribution of the 4q or 10q telomeres (red signal) and the whole X chromosome (green signal) in T1X cells after transfection of CTCF siRNA by 3D-FISH as described earlier (the blue signal corresponds to Lamin B). We do not observe a redistribution of the FISH signal for the 4q and 10q probes (panel A) or X chromosome territories (D) in cells transfected with siRNA compared with the control, suggesting that the reduced CTCF expression does not globally perturb the distribution of these chromosomes inside the nuclear space as observed for the D4Z4-tagged telomeres. For 4q and 10q distributions, we compared mock and knock-down conditions with the Mann–Whitney statistical test. The numbers of X territories associated to the Lamin B signal in these conditions were compared with the Fisher exact test. Download figure Download PowerPoint The multimerization of D4Z4 suppresses peripheral localization D4Z4 is repeated in tandem at several chromosomal loci including the 4q35 region linked to FSHD. Moreover, we demonstrated earlier that the binding of CTCF to D4Z4 is lost on multimerization of the repeat (Ottaviani et al, 2009) and that the positioning activity of D4Z4 is CTCF-dependent. Hence, we hypothesized that localization of a telomere-containing multiple D4Z4 repeats would be more internal and explored whether the multimerization alters D4Z4 perinuclear positioning activity. For this purpose, we transfected linearized DNA containing eight copies of the D4Z4 sequence abutting a telomere seed (T8X construct). As observed with the other telomere seeding plasmids containing 0 or 1 D4Z4 sequence, 94% of integrated T8X constructs form a de novo telomere (Supplementary Table S1) and the number of repeats remains stable after long-term culture (Supplementary Figure S5). As we hypothesized, T8X harbours the same subnuclear distribution than the D4Z4-less construct (T construct) and is more internal than telomeres carrying a single D4Z4 sequence (T1X construct) (Figure 4A and B). Interestingly, this loss of peripheral localization on multimerization correlates with a decrease in CTCF (Ottaviani et al, 2009) and A-type Lamins (Supplementary Figure S6) binding confirming the importance of CTCF for the peripheral positioning activity of D4Z4. Figure 4.Loss of CTCF binding upon D4Z4 multimerization abrogates peripheral tethering. (A) The position of the FISH signal was scored as described. The mean values±s.d. (y-axis) are presented for different populations compared with the Kruskal–Wallis statistic test (P<2.2 × 10−16, n=number of interphase nuclei). Multiple pairwise comparisons corrected for 5% FDR define four independent groups (identified by *; **; ***; **** above the brackets). (B) Mean positioning of the FISH signal for constructs with different number of D4Z4 from the centre (0%) to the outer (100%) edge of the nucleus. Download figure Download PowerPoint The fact that the wild-type 4q telomere is not delocalized on CTCF knock-down in our cells together with the particular behaviour of the 4q35 locus, which remains at the nuclear periphery even in FSHD patients with only one copy of D4Z4 (Masny et al, 2004; Tam et al, 2004), suggests the existence of at least two Lamins-dependent pathways for the perinuclear targeting of the 4q35 region. The first one is CTCF-independent and likely acts through a sequence located upstream of the D4Z4 array on the 4q35 region, as suggested earlier (Masny et al, 2004) (Figure 5A). According to our results, the second pathway might be linked to a gain of function of CTCF in patients carrying a single or a weakly oligomerized D4Z4 array (Ottaviani et al, 2009). In this configuration, a region located upstream of D4Z4 (Masny et al, 2004; Guelen et al, 2008; Petrov et al, 2008) might cooperate with the remaining D4Z4 repeats bound to CTCF to tighten the bonds between the corresponding region and the nucleus rim (Figure 5B). Consistent with the existence of two positioning elements at the 4q35 end, the proximal 4q35 FISH probe (D4S139) that hybridizes 215 kb upstream of the region containing the repeats is more peripheral than the D4Z4 array in normal cells (Masny et al, 2004) where CTCF is absent (Ottaviani et al, 2009). The putative existence of another region upstream of D4Z4 mediating perinuclear positioning and the inability of long D4Z4 arrays to bind CTCF and A-type Lamins in normal cells might also explain why the D4Z4 repeats alone do not tether the 10q26 telomere to the rim of the nucleus (Masny et al, 2004) and why LMNA knock-down does not affect the 4q35 positioning. Figure 5.Model for the tethering of the 4q35 locus at the nuclear periphery. (A) In normal cells, D4Z4 repeats are methylated (van Overveld et al, 2005) and not bound by CTCF (Ottaviani et al, 2009). On the basis of our data and those from Masny et al (2004); Tam et al (2004); Guelen et al (2008), we propose that this subtelomeric region is attached to the periphery through a Lamin-A-dependent tethering site localized outside the D4Z4 array, likely on the centromere side. (B) A model for the tethering of 4q35 at the nuclear periphery in FSHD cells. The contraction of D4Z4 allows the binding of CTCF (Ottaviani et al, 2009) and changes the functional organization of the 4q35 region. The D4Z4 repeat
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