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A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

2011; Royal Society of Chemistry; Volume: 47; Issue: 26 Linguagem: Inglês

10.1039/c1cc10442j

1364-548X

Zhuoxuan Lu, Demin Duan, Rong Cao, Liming Zhang, Kexiao Zheng, Jiang Li,

Advanced biosensing and bioanalysis techniques

A rapid and reverse transcription-free real-time PCR microRNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.