Location of rRNA genes in three inbred strains of rat and suppression of rat rRNA activity in rat-human somatic cell hybrids
1979; Elsevier BV; Volume: 119; Issue: 2 Linguagem: Inglês
10.1016/0014-4827(79)90368-9
ISSN1090-2422
AutoresRamana Tantravahi, D.A. Miller, G.G. D’Ancona, Carlo M. Croce, O. J. Miller,
Tópico(s)Animal Genetics and Reproduction
ResumoNucleolus organizer regions (NORs) of rat chromosomes were stained by the Ag-AS method. The Ag-NORs were found on chromosomes 3, 11 and 12 in the ACI, Wistar Brown and Wistar Lewis inbred strains of rat. The size of the Ag-NOR on each pair of chromosomes varied from strain to strain. Rat-human somatic hybrid cells that retained human and lost some of the rat chromosomes had no Ag-NOR on rat chromosomes 3, 11 or 12. Since NORs can be Ag-stained only if their 18 + 28S rRNA genes are active, the activity of the rat rRNA genes must have been suppressed in the hybrid cells.
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