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Purification and characterization of pancreatic colipase from the dogfish (Squalus acanthius)

1984; Elsevier BV; Volume: 789; Issue: 2 Linguagem: Inglês

10.1016/0167-4838(84)90200-0

1878-1454

Berit Sternby, Åke Engström, Ulf Hellman,

Protein Structure and Dynamics

Pure colipase from dogfish (Squalus acanthius) was obtained from an extract of pancreatic gland. It has a high isoelectric point (10.2) and the molecular weight was calculated to be 9108–9383. The N-terminal sequence was shown to be Gly-Leu-Phe-Leu-Asn-Leu-Ser-Ala-Gly-Glu-Leu-Cys-Val-Gly-Ser-Phe-Gln-Cys-Lys-Ser-Ser-Cys-Cys-Gln-Arg-Glu-Thr-Gly-Leu-Ser-Leu-Ala-Arg-Cys-Ala-. This sequence shows great homology with colipases from man, horse, pig and hen. There were indications of the existence of a proform of dogfish colipase. The propeptide was found to be Ala-Pro-Glu-Arg.