Produção Nacional
Revisado por pares
Linezolid-resistant Staphylococcus haemolyticus and Staphylococcus hominis: single and double mutations at the domain V of 23S rRNA among isolates from a Rio de Janeiro hospital
2014; Elsevier BV; Volume: 80; Issue: 4 Linguagem: Inglês
10.1016/j.diagmicrobio.2014.09.011
ISSN1879-0070
AutoresRaiane Cardoso Chamon, Natalia Lopes Pontes Iório, Fernanda Sampaio Cavalcante, Cristiane R. S. Teodoro, Ana Paula Chaves de Oliveira, Fernanda Maria Machado Maia, Kátia Regina Netto dos Santos,
Tópico(s)Bacterial Identification and Susceptibility Testing
ResumoIn this work, the molecular and phenotypic antimicrobial resistance and clonal diversity of 10 linezolid-resistant Staphylococcus spp. isolates were investigated. The 7 Staphylococcus haemolyticus isolates presented Staphylococcal cassete chromosome mec (SCCmec) V and belonged to the same pulsed-field gel electrophoresis pulsotype. Their MICs for oxacillin, vancomycin, and linezolid were ≥256 μg/mL, 1–4 μg/mL, and 8–16 μg/mL, respectively. The 3 S. hominis presented MIC values 32 to >256 μg/mL, 2–4 μg/mL, and 12–24 μg/mL, and all carried the nontypeable SCCmec (ccr1 + mecA class) and belonged to 2 different genotypes. The cfr gene was not found, but the mutation G2603T was detected in S. haemolyticus and C2190T and G2603T in Staphylococcus hominis in 23S rRNA. This study demonstrates the spread of a linezolid-resistant S. haemolyticus genotype and, for the first time, describes the mutation C2190T among S. hominis isolates with a double mutation in Brazil.
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