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The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing

1981; Oxford University Press; Volume: 9; Issue: 12 Linguagem: Inglês

10.1093/nar/9.12.2871

1362-4962

Richard C. Gardner, Alan J. Howarth, Peter Hahn, Marianne Brown-Luedi, Robert J. Shepherd, Joachim Messing,

Plant and Fungal Interactions Research

We have determined the complete primary structure (8031 base pairs) of an infectious clone of cauliflower mosaic virus strain CM1841. The sequence was obtained using the strategy of cloning shotgun restriction fragments in the sequencing vector M13mp7. Comparison of the CM1841 sequence with that published for another CaMV strain (Strasbourg) reveals 4.4% changes, mostly nucleotide substitutions with a few small insertions and deletions. The six open reading frames in the sequence of the Strasbourg isolate are also present in CM1841.