Portal de Periódicos da CAPES

Development of Immunochromatographic Assay for the On-site Detection of Salbutamol

2009; Taylor & Francis; Volume: 30; Issue: 4 Linguagem: Inglês

10.1080/15321810903188284

1532-4230

Yaowapa Khamta, Mookda Pattarawarapan, Sawitree Nangola, Chatchai Tayapiwatana,

Veterinary Pharmacology and Anesthesia

Abstract Abstract Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80 ng/mL of salbutamol in PBS. The method can be useful as an "on-site" screening procedure for detection of salbutamol. Keywords: Antibodies β-agonistsELISAGold colloidImmunochromatographic strip testSalbutamol ACKNOWLEDGMENTS This work was supported by The Commission on Higher Education, Minister of Education, Thailand and i + MED Laboratories Company Limited, Thailand. The authors would like to thank Dr. Dale E. Taneyhill for proofreading the manuscript and advice.