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Composição química e atividade anticolinesterásica de uma fração ativa do extrato de folhas de Citrus limon (L.) Burm

2013; Brazilian Chemical Society; Volume: 36; Issue: 9 Linguagem: Inglês

10.1590/s0100-40422013000900017

ISSN

1678-7064

Autores

Rusbene Bruno Fonseca de Carvalho, Antônia Amanda Cardoso de Almeida, Rivelilson Mendes de Freitas, Luciano S. Lima, Juceni P. David, Jorge M. David, Chistiane Mendes Feitosa,

Tópico(s)

Allelopathy and phytotoxic interactions

Resumo

Citrus limon (L.) Burm LEAVES.This paper describes the chemical composition and acetylcholinesterase inhibition of an active chromatographic fraction of the EtOAc extract of Citrus limon leaves.The composition of the active fraction presented a mixture of two coumarins, 5,8-dimethoxy-psoralen and 5,7-dimethoxycoumarin, identified by ¹H and ¹³C NMR data analysis, including DEPT, COSY, HMBC and HSQC experiments.It was also demonstrated that this mixture presents qualitative and quantitative AChE inhibition.In vitro studies indicated a CE50 value of 340 µg/mL with 95% of confidence.In vivo studies (10 and 25 mg/kg) revealed inhibition of 30.09 and 30.06% of AChE activity in relation to neostigmine, respectively.

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