Produção Nacional
Revisado por pares
Bacterial Cellulose-Hydroxyapatite Nanocomposites for Bone Regeneration
2011; Hindawi Publishing Corporation; Volume: 2011; Linguagem: Inglês
10.1155/2011/175362
ISSN1687-8795
AutoresSybele Saska, Hernane da Silva Barud, Ana Maria Minarelli Gaspar, Reinaldo Marchetto, Sidney J. L. Ribeiro, Younès Messaddeq,
Tópico(s)biodegradable polymer synthesis and properties
ResumoThe aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl(2) followed by Na(2)HPO(4). BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40%-50% of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration.
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