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An Old Method for Good New Cells

2009; Elsevier BV; Volume: 41; Issue: 4 Linguagem: Inglês

10.1016/j.transproceed.2009.03.078

1873-2623

Giuseppe Damiano, Maria Concetta Gioviale, C. Lombardo, Attilio Ignazio Lo Monte,

Xenotransplantation and immune response

The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).