Comparison of the Anthrone Reagent and a Copper-Reduction Method for Determining Blood Sugar in Calves
1961; Elsevier BV; Volume: 44; Issue: 11 Linguagem: Inglês
10.3168/jds.s0022-0302(61)90022-4
ISSN1529-9066
AutoresH.W. Colvin, John T. Attebery, J.T. Ivy,
Tópico(s)Microbial infections and disease research
ResumoProtein-free filtrates from 1,500 samples of blood from 12 calves were prepared according to ~olin-Wu.An aliquot of each filtrate was analyzed for blood sugar by both the anthrone reagent and the Somogyi-Nelson technique.The anthrone procedure is as follows: Pipet i ml. of sample filtrate, blank, or standard into a test tube and chill; add i0 ml. of ice-cold 0.05% anthrone dissolved in 72% sulfuric acid to each test tube while immersed in ice water and mix.After equalizing" the temperature of the tubes in a tap-water bath, place them in a boiling water bath for 10 rain.; cool in a running tap-water bath.Pour the reactants into matched spectrophotometer cuvettes and read at a wavelength of 620 m~ after aging for one-half hour in the dark.Statistical analysis indicated that the difference between the two techniques was highly significant.The anthrone values were less than 5% greater.The correlation coefficient between the results of the two methods was 0.94.The inherent error in both methods was the same, as indicated by almost identical standard deviations.
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