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Enzyme histochemistry of osteogenic sarcoma, chondrosarcoma, and giant-cell lesions in jawbones

1968; Elsevier BV; Volume: 26; Issue: 1 Linguagem: Inglês

10.1016/0030-4220(68)90229-6

1878-2175

M. Mori, Masakatsu Fukuda, Shinji Tsukamoto, Maki Takeda, Junichiro Arita, Masaaki Morishita,

Cancer, Hypoxia, and Metabolism

Distribution patterns of hydrolytic and oxidative enzymes were studied histochemically in osteogenic sarcoma, including giant-cell lesions, and chondrosarcoma which developed in mandibular and maxillary bones. Representative blocks were resected from fresh specimens and cut serially in a cryostat with or without the EDTA neutral decalcifying reagent at 4° C. Tumor cells in osteogenic sarcoma and chondrosarcoma were characterized by the presence of marked activity for alkaline and acid phosphatase as observed in the normal skeletal tissue. Giant tumor cells showed high activity for acid phosphatase, succinic dehydrogenase, and certain NAD-dependent dehydrogenases, as was the case with osteoclasts under physiologic conditions. Enzymatic stainabilities in osteogenic sarcoma and chondrosarcoma were generally high in lactic dehydrogenase and low in succinic dehydrogenase. These findings were similar to those in malignant tumors of human origin. NADP-dependent dehydrogenases, glucose-6-phosphate, and isocitric dehydrogenase activities revealed positive results in osteogenic tumor cells as observed in osteogenic cells in a healing bone fracture. In spite of decalcification, oxidative enzymes participating in many metabolic pathways in osteogenic tumors could be histochemically demonstrated in cryostat sections, as in tumors induced in oral regions.