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Multiple molecular species of cytoplasmic DNA polymerase from calf thymus

1974; Elsevier BV; Volume: 353; Issue: 4 Linguagem: Inglês

10.1016/0005-2787(74)90052-5

1879-3002

Shonen Yoshida, Toyoko Kondo, Teruo Ando,

Plant Genetic and Mutation Studies

Cytoplasmic DNA polymerase preparation from calf thymus was separated into three peaks on DEAE-cellulose column. All these three species preferred activated DNA to denatured DNA and were distinguished by their template specificities, isoelectric points, and the sedimentation rates on the sucrose gradients. Sedimentation rates of these three species lay between 6.7 and 8.5 (Mr 130 000–200 000). These three species were further dissociated into active smaller molecules (Mr 90 000 and 50 000–60 000) under certain conditions, such as a treatment with 2 M urea. Small active molecular weight species were also obtained by gel filtration on Sephadex G-200. These results suggest the existence of small active subunits in large DNA polymerase species in the cytoplasm of calf thymus.