A study of hemoglobin in antarctic fishes: purification and characterization of hemoglobins from four species
1988; Elsevier BV; Volume: 90; Issue: 3 Linguagem: Inglês
10.1016/0305-0491(88)90304-5
ISSN0305-0491
Autores Tópico(s)Hemoglobinopathies and Related Disorders
Resumo1. The number of hemoglobin components and their electrophoretic mobility has been investigated in hemolysates from several species of Nototheniidae, Harpagiferidae, Bathydraconidae, Zoarcidae and Rajidae. 2. In the seven nototheniid species, a major component (80–90%) and a second, more anodal one (5–15%) were detected, together with additional, minor components (less than 1%) in some species. 3. A single homoglobin was observed in the other cases, with the exception of four components in Zoarcidac. 4. The two main hemoglobins of three nototheniid species and the single component from the bathydraconid have been purified and obtained in crystalline form. 5. The oxygen-binding properties in erythrocytes, “stripped” hemolysates and purified hemoglobins from these four species appeared to be finely regulated by pH and allosteric effectors.
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