Portal de Periódicos da CAPES

Interaction of allopurinol with human blood

1982; Elsevier BV; Volume: 31; Issue: 10 Linguagem: Inglês

10.1016/0006-2952(82)90501-9

1873-2968

George Thomas, Stuart Feldman, William G. Kramer,

Folate and B Vitamins Research

Fresh and stored erythrocytes from normal and ITP-pyrophosphohydrolase (ITP-ase, EC 3.6.1.19) deficient individuals were incubated with hypoxanthine, guanine, allopurinol, and inosine. Differences in the purine metabolism between the normal and the ITP-ase deficient erythrocytes were observed only in the IMP-ITP cycle. Hypoxanthine, guanine and allopurinol were converted to nucleotides at the same rate. Hypoxanthine (2.5 μmol/l) inhibited the salvage of allopurinol (40 μmol/l). A slow decrease (0.7%/day) in salvage rate was observed in both types of cells upon storage at + 4 ° C.Erythrocyte ITP-ase activity was measured in a reference sample group of 48 healthy volunteers. Two distinct groups were found with mean activities equal to 48.3 ± 13.1 nkat/g Hb (χ ± SD, n = 38) and 11.4 ± 4.3 nkat/g Hb (n = 10). In two previously selected subjects, the ITP-ase activity was 0.2 and 2.4 nkat/g Hb. A hypothetical genetic mechanism is discussed.The maximal energy turnover in the IMP-ITP cycle during hypoxanthine incubation was found to be < 10% of the basal erythrocyte energy turnover.