MiR-150 Controls B Cell Differentiation by Targeting the Transcription Factor c-Myb
2007; Cell Press; Volume: 131; Issue: 1 Linguagem: Inglês
10.1016/j.cell.2007.07.021
ISSN1097-4172
AutoresChangchun Xiao, Dinis Pedro Calado, Gunther R. Galler, To‐Ha Thai, Heide Christine Patterson, Jing Wang, Nikolaus Rajewsky, Timothy P. Bender, Klaus Rajewsky,
Tópico(s)Immune Cell Function and Interaction
ResumoSummary MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.
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