Portal de Periódicos da CAPES

Synaptic localization of receptor-type protein tyrosine phosphatase ζ/β in the cerebral and hippocampal neurons of adult rats

2005; Elsevier BV; Volume: 1050; Issue: 1-2 Linguagem: Inglês

10.1016/j.brainres.2005.05.047

1872-6240

Noriko Hayashi, Atsuhiko Oohira, Seiji Miyata,

Glycosylation and Glycoproteins Research

Receptor-type protein tyrosine phosphatase (RPTP) ζ/β is a nervous tissue-specific chondroitin sulfate proteoglycan. In this study, we investigated the immunohistochemical localization of RPTPζ/β in adult rat cerebral cortex and hippocampus at light and electron microscopic levels. Double labeling immunofluorescence microscopy revealed that the immunoreactivity of RPTPζ/β was observed at MAP2-positive dendrites and PSD-95-positive spines of pyramidal neurons in the cerebral cortex and hippocampus. Electron microscopic observation demonstrated a strong immunoreactivity of RPTPζ/β at the postsynaptic membrane of dendritic spines and shafts, and its moderate immunoreactivity at the dendritic membrane. In cultured cortical neurons, the immunoreactivity of RPTPζ/β was observed at some of PSD-95-positive spines. These results demonstrate that RPTPζ/β is localized mainly at the postsynaptic membrane of pyramidal neurons in adult cerebral cortex and hippocampus.