Phospholipid composition modifications influence phospholipase A2 activity in rat liver plasma membranes
1986; Elsevier BV; Volume: 18; Issue: 10 Linguagem: Inglês
10.1016/0020-711x(86)90076-5
ISSN1878-6014
AutoresAlbena Momchilova, Diana Petkova, Kamen Koumanov,
Tópico(s)Molecular Sensors and Ion Detection
ResumoThe influence of the phospholipid composition and the physico-chemical properties of rat liver plasma membranes on the activity of membrane-bound phospholipase A2 has been investigated. The plasma membrane composition was modified by the aid of exogenous phospholipases A2, C and D, and by butanol treatment. The partially delipidated membranes thus obtained were enriched with different phospholipids. The steady-state fluorescent anisotropy of 1,6-diphenyl-1,3,5-hexatriene and the lipid order parameter-SDPH in the modified membranes were calculated. It was established that the activity of the membrane-bound phospholipase A2 was higher in rigid membranes and was decreased when the membrane lipid bilayer was fluidized.
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