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In Vitro Anti- Candida Activity of Lidocaine and Nitroglycerin: Alone and Combined

2012; Hindawi Publishing Corporation; Volume: 2012; Linguagem: Inglês

10.1155/2012/727248

1098-0997

Ana Palmeira‐de‐Oliveira, A.R. Ramos, Carlos Gaspar, Rita Palmeira‐de‐Oliveira, P. Gouveia, José Martinez‐de‐Oliveira,

Antimicrobial Resistance in Staphylococcus

The aim of this work was to study the anti- Candida activity of lidocaine and nitroglycerin alone and in combination. Ten Candida strains were included, corresponding to 1 collection type strain (ATCC 10231) and 9 clinical isolates: 4 C. albicans , 2 C. glabrata , 1 C. tropicalis , 1 C. krusei , and 1 C. parapsilosis . The CLSI reference M27-A3 micromethod was used to determine the anti- Candida activity of the drugs alone; minimal inhibitory and lethal concentrations were determined. The classic checkboard technique was used to determine the activity of combined drugs. Lidocaine fungicidal effect was dosedependent. Nitroglycerin exhibited a higher effect. The drugs combination resulted in a reduction of the inhibitory concentration, corresponding to an additive effect. In conclusion, both drugs exhibited an interesting anti- Candida activity. The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis.