Detection of Individual p53‐Autoantibodies by Using Quenched Peptide‐Based Molecular Probes
2002; Wiley; Volume: 41; Issue: 24 Linguagem: Inglês
10.1002/anie.200290044
ISSN1521-3773
AutoresHannes Neuweiler, Andreas Schulz, Andrea C. Vaiana, Jeremy C. Smith, Sepp Kaul, J. Wolfrum, Markus Sauer,
Tópico(s)Advanced Biosensing Techniques and Applications
ResumoSingle-molecule spectroscopy for cancer diagnostics: Short dye-labeled peptide epitopes derived from human p53 show strong fluorescence quenching owing to efficient electron transfer from a tryptophan residue to the fluorophore (1, see picture). The specific recognition of the peptide epitope by the antibody induces a change in the conformation which is accompanied by a strong increase in fluorescence intensity. The fluorescing antibody–peptide complex can be detected at the single-molecule level even in slightly diluted serum samples by using confocal fluorescence microscopy.
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