Multicolor Luciferase Assay System: One-Step Monitoring of Multiple Gene Expressions with a Single Substrate
2005; Future Science Ltd; Volume: 38; Issue: 6 Linguagem: Inglês
10.2144/05386st03
ISSN1940-9818
AutoresYoshihiro Nakajima, Takuma Kimura, Kazunori Sugata, Toshiteru Enomoto, Atsushi Asakawa, Hidehiro Kubota, Masaaki Ikeda, Yoshihiro Ohmiya,
Tópico(s)Biosensors and Analytical Detection
ResumoReporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of more than one gene cannot be monitored simultaneously by this system because of one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay system in which three luciferases that emit green, orange, and red light with a single substrate are used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply and rapidly monitor multiple gene expressions in a one-step reaction.
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