In vitro biosynthesis of the cyanogenic glucoside taxiphyllin in Triglochin maritima
1980; Elsevier BV; Volume: 203; Issue: 2 Linguagem: Inglês
10.1016/0003-9861(80)90235-0
ISSN1096-0384
AutoresWolfgang Hösel, Adolf Nahrstedt,
Tópico(s)Cassava research and cyanide
ResumoWhen l-tyrosine and NADPH were incubated with a microsomal fraction from Triglochin maritima seedlings, 4-hydroxymandelonitrile (measured as 4-hydroxybenzaldehyde and HCN) and 4-hydroxyphenylacetonitrile were synthesized. Taxiphyllin, a cyanogenic glucoside occurring in this plant, was formed when UDPglucose and a soluble protein fraction from T. maritima, were also included in the assay. The above glucosyl transferase activity also produced taxiphyllin when incubated with R,S-4-hydroxymandelonitrile and UDPglucose. No trace of dhurrin, the enantiomer of taxiphyllin, was detected. The highest microsomal activity was found in 5- to 6-day-old etiolated seedlings. It was absolutely necessary to remove all seed coats from the seedlings in order to obtain an active microsomal fraction.
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