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Studies of the incorporation in vitro of deoxyribonucleic acid by cells of the Ehrlich-Lettré ascites carcinoma

1965; Elsevier BV; Volume: 103; Issue: 3 Linguagem: Inglês

10.1016/0005-2787(65)90136-x

1879-3002

Stanley Meizel, Ernest Kay,

DNA Repair Mechanisms

A study has been made of the incorporation in vitro of labeled DNA into the cells of the Ehrlich-Lettré carcinoma. Analysis of the specific activity ratios of adenine/thymine of the experimental DNA used in the incorporation experiments showed that the ratio remained relatively constant. This would suggest that the DNA is highly polymerized during its process of incorporation into the nucleus. There was some evidence of breakdown during the process of incorporation and the incorporation was at a maximum during the first 15 min of the incubation. A study of some metabolic events during the incorporation showed that DNA incorporation was greater in the absence of glucose than in the presence of 2·10−2M glucose. Maximum incorporation of DNA occurred with 2·10−4M glucose. NaF inhibited incorporation and 2,4-dinitrophenol stimulated incorporation, but both together produced a greater inhibition than NaF alone. This suggests that both respiration and glycolysis are involved in the energy requirements for incorporation of the highly polymerized DNA into these cells.