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Damage induced by stannous chloride in plasmid DNA

2000; Elsevier BV; Volume: 116; Issue: 1-2 Linguagem: Inglês

10.1016/s0378-4274(00)00213-7

1879-3169

José Carlos Pelielo de Mattos, F.J.S. Dantas, Roberto J.A.C. Bezerra, Mário Bernardo-Filho, Januário B. Cabral‐Neto, Claudia Lage, Álvaro C. Leitão, Adriano Caldeira-de-Araújo,

DNA Repair Mechanisms

Stannous chloride (SnCl(2)) is widely used in daily human life, for example, to conserve soft drinks, in food manufacturing and biocidal preparations. In nuclear medicine, stannous chloride is used as a reducing agent of Technetium-99m, a radionuclide used to label different cells and molecules. In spite of this, stannous chloride is able to generate reactive oxygen species (ROS) which can damage DNA. In this work, plasmid DNA (pUC 9.1) was incubated with SnCl(2) under different conditions and the results analyzed through DNA migration in agarose gel electrophoresis. Our data reinforce the powerful damaging effect induced by stannous ion and suggest that this salt can play a direct role in inducing DNA lesions.