Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Nematicidal action of chitinases produced by the fungus Monacrosporium thaumasium under laboratorial conditions

2014; Taylor & Francis; Volume: 25; Issue: 3 Linguagem: Inglês

10.1080/09583157.2014.979133

1360-0478

Filippe Elias de Freitas Soares, José Humberto de Queiróz, Jackson Víctor de Araújo, Paula Viana Queiroz, Angélica de Souza Gouveia, Emy Hiura, Fábio Ribeiro Braga,

Entomopathogenic Microorganisms in Pest Control

AbstractNematophagous fungi produce chitinases that may be important in the process of infection of eggs and larvae of nematodes. This study aimed to produce, purify, characterise and test the nematicidal action of extracellular chitinases produced by Monacrosporium thaumasium on Panagrellus redivivus. Mycelia from M. thaumasium were used to inoculate a solid medium for chitinase production. The enzymes were purified using a specific technique of adsorption for chitinases. The chitinase activity was determined at different pHs and temperatures. NF34 produced two distinct chitinases (27 and 30 kDa). After 72 hours, these enzymes provided a significant reduction (80%; p < 0.01) of the number of P. redivivus larvae, compared to control. It was shown that isolate NF34 produced chitinases with nematicidal activity. Thus, other experimental designs on geohelminths or even arthropods that transmit diseases may become a new aspect of the field of study of biological control using predatory nematophagous fungi.Keywords: nematophagous fungiMonacrosporium thaumasiumchitinase AcknowledgementThe authors would like to thank CNPq scholarship to JVdA, FAPEMIG and CAPES for the financial support and grant concession.