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A Trans ‐Acting Ketoreductase in Biosynthesis of a Symmetric Polyketide Dimer SIA7248

2013; Wiley; Volume: 14; Issue: 6 Linguagem: Inglês

10.1002/cbic.201300068

1439-7633

Yi Zou, Haixing Yin, Dekun Kong, Zixin Deng, Shuangjun Lin,

Marine Sponges and Natural Products

ChemBioChemVolume 14, Issue 6 p. 679-683 Communication A Trans-Acting Ketoreductase in Biosynthesis of a Symmetric Polyketide Dimer SIA7248 Yi Zou, Yi Zou State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorHaixing Yin, Haixing Yin State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorDekun Kong, Dekun Kong State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorProf. Dr. Zixin Deng, Prof. Dr. Zixin Deng State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorProf. Dr. Shuangjun Lin, Corresponding Author Prof. Dr. Shuangjun Lin linsj@sjtu.edu.cn State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this author Yi Zou, Yi Zou State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorHaixing Yin, Haixing Yin State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorDekun Kong, Dekun Kong State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorProf. Dr. Zixin Deng, Prof. Dr. Zixin Deng State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this authorProf. Dr. Shuangjun Lin, Corresponding Author Prof. Dr. Shuangjun Lin linsj@sjtu.edu.cn State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030 (P.R. China)Search for more papers by this author First published: 15 March 2013 https://doi.org/10.1002/cbic.201300068Citations: 21Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract New assembly line, new compound: SIA7248, a new symmetric macrolide, was isolated from a marine-derived Streptomyces strain. Bioinformatic analyses of the identified biosynthetic gene cluster (sia) for SIA7248 suggested a polyketide biosynthesis utilizing an iteratively trans-acting ketoreductase (KR). We characterized SiaM as a trans-KR to catalyse reductions of various β-ketoacyl-thioesters with D-stereospecificity. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description cbic_201300068_sm_miscellaneous_information.pdf2.2 MB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume14, Issue6April 15, 2013Pages 679-683 RelatedInformation