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Identification of a major cytokinin in coconut milk

1995; Birkhäuser; Volume: 51; Issue: 11 Linguagem: Inglês

10.1007/bf01946921

0014-4754

Hiroshi Kobayashi, Naoko Morisaki, Yoshitaka Tago, Yosuke Hashimoto, Shun Iwasaki, Emiko Kawachi, Ryuji Nagata, Koichi Shudo,

Food Chemistry and Fat Analysis

A major cytokinin found in coconut milk was isolated by using the tobacco callus growth-promoting assay as a guide during purification. The structure of the factor was determined to be 14-O-(3-O-[beta-D-galactopyranosyl-(1-->2)-alpha-D-galactopyranosyl-(1-- >3)- alpha-L-arabinofuranosyl]-4-O-(alpha-L-arabinofuranosyl)-beta-D- galactopyranosyl)-trans-zeatin riboside [G3A2-ZR] by various NMR techniques, including heteronuclear multiple bond connectivity by 2D multiple quantum NMR (HMBC), as well as mass spectroscopy and sugar analysis. The optimum concentration of G3A2-ZR for cytokinin activity in the tobacco callus assay was estimated to be 5 x 10(-6) M, so that G3A2-ZR is one order of magnitude more potent than 1,3-diphenylurea and one order less potent than zeatin riboside. At least 20% of the cytokinin activity of coconut milk could be attributed to G3A2-ZR.