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Reconstruction of glutamine synthetase using computer averaging

1978; Elsevier BV; Volume: 3; Linguagem: Inglês

10.1016/s0304-3991(78)80038-2

1879-2723

Joachim Frank, Walter Goldfarb, David Eisenberg, Timothy S. Baker,

Mass Spectrometry Techniques and Applications

The axial projection of the glutamine synthetase molecule has been reconstructed from electron micrographs of a stained preparation by using a new method of correlation search and averaging. The average over 50 individual molecules appears as a radial pattern with sixfold symmetry. The handedness evident in the average is attributed to nonuniformity of the negative stain.