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Antibacterial activity of chitosan grafting nisin: Preparation and characterization

2015; Elsevier BV; Volume: 91-92; Linguagem: Inglês

10.1016/j.reactfunctpolym.2015.04.009

1873-166X

Xiaoming Zhu, Huan Wu, Jing Yang, Jun Tong, Jiayan Yi, Zhihai Hu, Jin Hu, Tan Wang, Lihong Fan,

Polysaccharides and Plant Cell Walls

Nisin grafted chitosan was prepared by using microbial transglutaminase as biocatalyst. The transglutaminase-catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The results revealed that the degree of substitution (DS) of nisin–chitosan could be controlled by adjusting the reaction time, the reaction temperature and the molar ratio of nisin to chitosan. And nisin–chitosan in different pH showed excellent solubility. In addition, in vitro antibacterial activity assessment, nisin–chitosan with the concentration of 0.008 mg/mL showed pronounced inhibitory effect against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Furthermore, L929 mouse fibroblasts were cultured with nisin–chitosan, and the methylthiazol tetrazolium (MTT) assay showed that nisin–chitosan with the concentration from 0.005 to 0.01 mg/mL displayed low toxicity. The results may contribute to finding the application of nisin–chitosan in pharmaceutical and food industry fields.