Portal de Periódicos da CAPES

Laccase-catalyzed conversion of natural and synthetic hormones from a municipal wastewater

2007; Elsevier BV; Volume: 41; Issue: 15 Linguagem: Inglês

10.1016/j.watres.2007.05.008

1879-2448

M Auriol, Youssef Filali-Meknassi, R.D. Tyagi, Craig D. Adams,

Electrochemical sensors and biosensors

The Trametes versicolor-derived laccase-catalyzed oxidation of natural estrogens (estrone—E1; 17β-estradiol—E2; and estriol—E3) and a synthetic estrogen (17α-ethinylestradiol—EE2) was studied in synthetic water and municipal wastewater to optimize the process for steroid estrogen removal in wastewater. The optimal pH for each studied steroid estrogen oxidation was approximately 6 in synthetic water. This research also focused on the wastewater matrix effect on developed enzymatic treatment. At pH 7.0 and 25±1 °C, the experiments showed that the laccase-catalyzed system for the removal of steroid estrogens was not significantly affected by the municipal wastewater matrix. Laccase activity of 20 U/ml was sufficient to achieve complete removal of studied steroid estrogens in both synthetic water and municipal wastewater. Moreover, 1-hydroxy-benzotriazole, when used as a mediator, improved laccase-catalyzed system efficiency, thus decreasing the overall cost of the enzymatic system.